Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.
The mechanism of arachidonic acid (AA)-induced LH release was characterized using sheep pituitary cells in primary culture permeabilized with Staphylococcal alpha-toxin. In intact cells, exogenous AA evoked release of LH in a manner which was partially dependent on extracellular Ca2+. At similar con...
Hauptverfasser: | , , , |
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Format: | Journal article |
Sprache: | English |
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1992
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_version_ | 1826294224389144576 |
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author | Kaye, P Van Der Merwe, P Millar, R Davidson, J |
author_facet | Kaye, P Van Der Merwe, P Millar, R Davidson, J |
author_sort | Kaye, P |
collection | OXFORD |
description | The mechanism of arachidonic acid (AA)-induced LH release was characterized using sheep pituitary cells in primary culture permeabilized with Staphylococcal alpha-toxin. In intact cells, exogenous AA evoked release of LH in a manner which was partially dependent on extracellular Ca2+. At similar concentrations, AA also caused cell permeabilization as monitored by efflux of [3H]2-deoxyglucose metabolites. In alpha-toxin-permeabilized cells where cytosolic Ca2+ was clamped at resting levels, AA retained its ability to cause LH release. Unlike the stimulation of exocytosis produced by Ca2+, phorbol ester or cyclic AMP, AA-evoked release was independent of ATP and was not inhibited by pretreatment with N-ethyl maleimide. These findings indicated that exogenous AA does not cause LH release by Ca2+ influx or mobilization or by activating protein kinase C. The results suggest that LH release induced by exogenous AA is probably due to its detergent-like properties, and does not represent true exocytosis. |
first_indexed | 2024-03-07T03:42:21Z |
format | Journal article |
id | oxford-uuid:be52a764-2bc3-4a63-8214-bdaaefd70d7e |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-07T03:42:21Z |
publishDate | 1992 |
record_format | dspace |
spelling | oxford-uuid:be52a764-2bc3-4a63-8214-bdaaefd70d7e2022-03-27T05:38:31ZArachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:be52a764-2bc3-4a63-8214-bdaaefd70d7eEnglishSymplectic Elements at Oxford1992Kaye, PVan Der Merwe, PMillar, RDavidson, JThe mechanism of arachidonic acid (AA)-induced LH release was characterized using sheep pituitary cells in primary culture permeabilized with Staphylococcal alpha-toxin. In intact cells, exogenous AA evoked release of LH in a manner which was partially dependent on extracellular Ca2+. At similar concentrations, AA also caused cell permeabilization as monitored by efflux of [3H]2-deoxyglucose metabolites. In alpha-toxin-permeabilized cells where cytosolic Ca2+ was clamped at resting levels, AA retained its ability to cause LH release. Unlike the stimulation of exocytosis produced by Ca2+, phorbol ester or cyclic AMP, AA-evoked release was independent of ATP and was not inhibited by pretreatment with N-ethyl maleimide. These findings indicated that exogenous AA does not cause LH release by Ca2+ influx or mobilization or by activating protein kinase C. The results suggest that LH release induced by exogenous AA is probably due to its detergent-like properties, and does not represent true exocytosis. |
spellingShingle | Kaye, P Van Der Merwe, P Millar, R Davidson, J Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide. |
title | Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide. |
title_full | Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide. |
title_fullStr | Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide. |
title_full_unstemmed | Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide. |
title_short | Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide. |
title_sort | arachidonic acid induced lh release is atp independent and insensitive to n ethyl maleimide |
work_keys_str_mv | AT kayep arachidonicacidinducedlhreleaseisatpindependentandinsensitivetonethylmaleimide AT vandermerwep arachidonicacidinducedlhreleaseisatpindependentandinsensitivetonethylmaleimide AT millarr arachidonicacidinducedlhreleaseisatpindependentandinsensitivetonethylmaleimide AT davidsonj arachidonicacidinducedlhreleaseisatpindependentandinsensitivetonethylmaleimide |