Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.

The mechanism of arachidonic acid (AA)-induced LH release was characterized using sheep pituitary cells in primary culture permeabilized with Staphylococcal alpha-toxin. In intact cells, exogenous AA evoked release of LH in a manner which was partially dependent on extracellular Ca2+. At similar con...

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Hauptverfasser: Kaye, P, Van Der Merwe, P, Millar, R, Davidson, J
Format: Journal article
Sprache:English
Veröffentlicht: 1992
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author Kaye, P
Van Der Merwe, P
Millar, R
Davidson, J
author_facet Kaye, P
Van Der Merwe, P
Millar, R
Davidson, J
author_sort Kaye, P
collection OXFORD
description The mechanism of arachidonic acid (AA)-induced LH release was characterized using sheep pituitary cells in primary culture permeabilized with Staphylococcal alpha-toxin. In intact cells, exogenous AA evoked release of LH in a manner which was partially dependent on extracellular Ca2+. At similar concentrations, AA also caused cell permeabilization as monitored by efflux of [3H]2-deoxyglucose metabolites. In alpha-toxin-permeabilized cells where cytosolic Ca2+ was clamped at resting levels, AA retained its ability to cause LH release. Unlike the stimulation of exocytosis produced by Ca2+, phorbol ester or cyclic AMP, AA-evoked release was independent of ATP and was not inhibited by pretreatment with N-ethyl maleimide. These findings indicated that exogenous AA does not cause LH release by Ca2+ influx or mobilization or by activating protein kinase C. The results suggest that LH release induced by exogenous AA is probably due to its detergent-like properties, and does not represent true exocytosis.
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spelling oxford-uuid:be52a764-2bc3-4a63-8214-bdaaefd70d7e2022-03-27T05:38:31ZArachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:be52a764-2bc3-4a63-8214-bdaaefd70d7eEnglishSymplectic Elements at Oxford1992Kaye, PVan Der Merwe, PMillar, RDavidson, JThe mechanism of arachidonic acid (AA)-induced LH release was characterized using sheep pituitary cells in primary culture permeabilized with Staphylococcal alpha-toxin. In intact cells, exogenous AA evoked release of LH in a manner which was partially dependent on extracellular Ca2+. At similar concentrations, AA also caused cell permeabilization as monitored by efflux of [3H]2-deoxyglucose metabolites. In alpha-toxin-permeabilized cells where cytosolic Ca2+ was clamped at resting levels, AA retained its ability to cause LH release. Unlike the stimulation of exocytosis produced by Ca2+, phorbol ester or cyclic AMP, AA-evoked release was independent of ATP and was not inhibited by pretreatment with N-ethyl maleimide. These findings indicated that exogenous AA does not cause LH release by Ca2+ influx or mobilization or by activating protein kinase C. The results suggest that LH release induced by exogenous AA is probably due to its detergent-like properties, and does not represent true exocytosis.
spellingShingle Kaye, P
Van Der Merwe, P
Millar, R
Davidson, J
Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.
title Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.
title_full Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.
title_fullStr Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.
title_full_unstemmed Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.
title_short Arachidonic acid-induced LH release is ATP-independent and insensitive to N-ethyl maleimide.
title_sort arachidonic acid induced lh release is atp independent and insensitive to n ethyl maleimide
work_keys_str_mv AT kayep arachidonicacidinducedlhreleaseisatpindependentandinsensitivetonethylmaleimide
AT vandermerwep arachidonicacidinducedlhreleaseisatpindependentandinsensitivetonethylmaleimide
AT millarr arachidonicacidinducedlhreleaseisatpindependentandinsensitivetonethylmaleimide
AT davidsonj arachidonicacidinducedlhreleaseisatpindependentandinsensitivetonethylmaleimide