Tissue-resident B cells orchestrate macrophage polarisation and function
B cells play a central role in humoral immunity but also have antibody-independent functions. Studies to date have focused on B cells in blood and secondary lymphoid organs but whether B cells reside in non-lymphoid organs (NLO) in homeostasis is unknown. Here we identify, using intravenous labeling...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Springer Nature
2023
|
| _version_ | 1826316100110909440 |
|---|---|
| author | Suchanek, O Ferdinand, JR Tuong, ZK Wijeyesinghe, S Chandra, A Clauder, A-K Almeida, LN Clare, S Harcourt, K Ward, CJ Bashford-Rogers, R Lawley, T Manz, RA Okkenhaug, K Masopust, D Clatworthy, MR |
| author_facet | Suchanek, O Ferdinand, JR Tuong, ZK Wijeyesinghe, S Chandra, A Clauder, A-K Almeida, LN Clare, S Harcourt, K Ward, CJ Bashford-Rogers, R Lawley, T Manz, RA Okkenhaug, K Masopust, D Clatworthy, MR |
| author_sort | Suchanek, O |
| collection | OXFORD |
| description | B cells play a central role in humoral immunity but also have antibody-independent functions. Studies to date have focused on B cells in blood and secondary lymphoid organs but whether B cells reside in non-lymphoid organs (NLO) in homeostasis is unknown. Here we identify, using intravenous labeling and parabiosis, a bona-fide tissue-resident B cell population in lung, liver, kidney and urinary bladder, a substantial proportion of which are B-1a cells. Tissue-resident B cells are present in neonatal tissues and also in germ-free mice NLOs, albeit in lower numbers than in specific pathogen-free mice and following co-housing with 'pet-store' mice. They spatially co-localise with macrophages and regulate their polarization and function, promoting an anti-inflammatory phenotype, in-part via interleukin-10 production, with effects on bacterial clearance during urinary tract infection. Thus, our data reveal a critical role for tissue-resident B cells in determining the homeostatic 'inflammatory set-point' of myeloid cells, with important consequences for tissue immunity. |
| first_indexed | 2024-12-09T03:39:46Z |
| format | Journal article |
| id | oxford-uuid:be80caf6-21a3-43a1-9530-64d24a21cd1d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:39:46Z |
| publishDate | 2023 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:be80caf6-21a3-43a1-9530-64d24a21cd1d2024-12-05T15:35:23ZTissue-resident B cells orchestrate macrophage polarisation and functionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:be80caf6-21a3-43a1-9530-64d24a21cd1dEnglishSymplectic ElementsSpringer Nature2023Suchanek, OFerdinand, JRTuong, ZKWijeyesinghe, SChandra, AClauder, A-KAlmeida, LNClare, SHarcourt, KWard, CJBashford-Rogers, RLawley, TManz, RAOkkenhaug, KMasopust, DClatworthy, MRB cells play a central role in humoral immunity but also have antibody-independent functions. Studies to date have focused on B cells in blood and secondary lymphoid organs but whether B cells reside in non-lymphoid organs (NLO) in homeostasis is unknown. Here we identify, using intravenous labeling and parabiosis, a bona-fide tissue-resident B cell population in lung, liver, kidney and urinary bladder, a substantial proportion of which are B-1a cells. Tissue-resident B cells are present in neonatal tissues and also in germ-free mice NLOs, albeit in lower numbers than in specific pathogen-free mice and following co-housing with 'pet-store' mice. They spatially co-localise with macrophages and regulate their polarization and function, promoting an anti-inflammatory phenotype, in-part via interleukin-10 production, with effects on bacterial clearance during urinary tract infection. Thus, our data reveal a critical role for tissue-resident B cells in determining the homeostatic 'inflammatory set-point' of myeloid cells, with important consequences for tissue immunity. |
| spellingShingle | Suchanek, O Ferdinand, JR Tuong, ZK Wijeyesinghe, S Chandra, A Clauder, A-K Almeida, LN Clare, S Harcourt, K Ward, CJ Bashford-Rogers, R Lawley, T Manz, RA Okkenhaug, K Masopust, D Clatworthy, MR Tissue-resident B cells orchestrate macrophage polarisation and function |
| title | Tissue-resident B cells orchestrate macrophage polarisation and function |
| title_full | Tissue-resident B cells orchestrate macrophage polarisation and function |
| title_fullStr | Tissue-resident B cells orchestrate macrophage polarisation and function |
| title_full_unstemmed | Tissue-resident B cells orchestrate macrophage polarisation and function |
| title_short | Tissue-resident B cells orchestrate macrophage polarisation and function |
| title_sort | tissue resident b cells orchestrate macrophage polarisation and function |
| work_keys_str_mv | AT suchaneko tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT ferdinandjr tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT tuongzk tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT wijeyesinghes tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT chandraa tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT clauderak tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT almeidaln tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT clares tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT harcourtk tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT wardcj tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT bashfordrogersr tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT lawleyt tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT manzra tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT okkenhaugk tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT masopustd tissueresidentbcellsorchestratemacrophagepolarisationandfunction AT clatworthymr tissueresidentbcellsorchestratemacrophagepolarisationandfunction |