In vivo cardiac 1H-MRS in the mouse.
The mouse is the predominant animal model to study the effect of gene manipulations. Imaging techniques to define functional effects on the heart caused by genomic alterations are becoming increasingly routine in mice, yet methods for in vivo investigation of metabolic phenotypes in the mouse heart...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2004
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| _version_ | 1797092268641878016 |
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| author | Schneider, J Tyler, D ten Hove, M Sang, A Cassidy, P Fischer, A Wallis, J Sebag-Montefiore, L Watkins, H Isbrandt, D Clarke, K Neubauer, S |
| author_facet | Schneider, J Tyler, D ten Hove, M Sang, A Cassidy, P Fischer, A Wallis, J Sebag-Montefiore, L Watkins, H Isbrandt, D Clarke, K Neubauer, S |
| author_sort | Schneider, J |
| collection | OXFORD |
| description | The mouse is the predominant animal model to study the effect of gene manipulations. Imaging techniques to define functional effects on the heart caused by genomic alterations are becoming increasingly routine in mice, yet methods for in vivo investigation of metabolic phenotypes in the mouse heart are lacking. In this work, cardiac 1H-MRS was developed and applied in mouse hearts in vivo using a single-voxel technique (PRESS). In normal C57Bl/6J mice, stability and reproducibility achieved by dedicated cardiac and respiratory gating was demonstrated by measuring amplitude and zero-order phase changes of the unsuppressed water signal. Various cardiac metabolites, such as creatine, taurine, carnitine, or intramyocardial lipids were successfully detected and quantified relative to the total water content in voxels as small as 2 microl, positioned in the interventricular septum. The method was applied to a murine model of guanidinoacetate N-methyltransferase (GAMT) deficiency, which is characterized by substantially decreased myocardial creatine levels. Creatine deficiency was confirmed noninvasively in myocardium of anesthetized GAMT-/- mice. This is the first study to report the application of cardiac 1H-MRS in mice in vivo. |
| first_indexed | 2024-03-07T03:43:44Z |
| format | Journal article |
| id | oxford-uuid:bebc6816-ea8b-4e32-806b-2118a0e07ae9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:43:44Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:bebc6816-ea8b-4e32-806b-2118a0e07ae92022-03-27T05:42:10ZIn vivo cardiac 1H-MRS in the mouse.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bebc6816-ea8b-4e32-806b-2118a0e07ae9EnglishSymplectic Elements at Oxford2004Schneider, JTyler, Dten Hove, MSang, ACassidy, PFischer, AWallis, JSebag-Montefiore, LWatkins, HIsbrandt, DClarke, KNeubauer, SThe mouse is the predominant animal model to study the effect of gene manipulations. Imaging techniques to define functional effects on the heart caused by genomic alterations are becoming increasingly routine in mice, yet methods for in vivo investigation of metabolic phenotypes in the mouse heart are lacking. In this work, cardiac 1H-MRS was developed and applied in mouse hearts in vivo using a single-voxel technique (PRESS). In normal C57Bl/6J mice, stability and reproducibility achieved by dedicated cardiac and respiratory gating was demonstrated by measuring amplitude and zero-order phase changes of the unsuppressed water signal. Various cardiac metabolites, such as creatine, taurine, carnitine, or intramyocardial lipids were successfully detected and quantified relative to the total water content in voxels as small as 2 microl, positioned in the interventricular septum. The method was applied to a murine model of guanidinoacetate N-methyltransferase (GAMT) deficiency, which is characterized by substantially decreased myocardial creatine levels. Creatine deficiency was confirmed noninvasively in myocardium of anesthetized GAMT-/- mice. This is the first study to report the application of cardiac 1H-MRS in mice in vivo. |
| spellingShingle | Schneider, J Tyler, D ten Hove, M Sang, A Cassidy, P Fischer, A Wallis, J Sebag-Montefiore, L Watkins, H Isbrandt, D Clarke, K Neubauer, S In vivo cardiac 1H-MRS in the mouse. |
| title | In vivo cardiac 1H-MRS in the mouse. |
| title_full | In vivo cardiac 1H-MRS in the mouse. |
| title_fullStr | In vivo cardiac 1H-MRS in the mouse. |
| title_full_unstemmed | In vivo cardiac 1H-MRS in the mouse. |
| title_short | In vivo cardiac 1H-MRS in the mouse. |
| title_sort | in vivo cardiac 1h mrs in the mouse |
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