Gene loops enhance transcriptional directionality
Eukaryotic genomes are extensively transcribed, forming both messenger RNAs (mRNAs) and noncoding RNAs (ncRNAs). ncRNAs made by RNA polymerase II often initiate from bidirectional promoters (nucleosome-depleted chromatin) that synthesize mRNA and ncRNA in opposite directions. We demonstrate that, by...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Association for the Advancement of Science
2012
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| _version_ | 1797092291766124544 |
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| author | Tan-Wong, S Zaugg, J Camblong, J Xu, Z Zhang, D Mischo, H Ansari, A Luscombe, N Steinmetz, L Proudfoot, N |
| author_facet | Tan-Wong, S Zaugg, J Camblong, J Xu, Z Zhang, D Mischo, H Ansari, A Luscombe, N Steinmetz, L Proudfoot, N |
| author_sort | Tan-Wong, S |
| collection | OXFORD |
| description | Eukaryotic genomes are extensively transcribed, forming both messenger RNAs (mRNAs) and noncoding RNAs (ncRNAs). ncRNAs made by RNA polymerase II often initiate from bidirectional promoters (nucleosome-depleted chromatin) that synthesize mRNA and ncRNA in opposite directions. We demonstrate that, by adopting a gene-loop conformation, actively transcribed mRNA encoding genes restrict divergent transcription of ncRNAs. Because gene-loop formation depends on a protein factor (Ssu72) that coassociates with both the promoter and the terminator, the inactivation of Ssu72 leads to increased synthesis of promoter-associated divergent ncRNAs, referred to as Ssu72-restricted transcripts (SRTs). Similarly, inactivation of individual gene loops by gene mutation enhances SRT synthesis. We demonstrate that gene-loop conformation enforces transcriptional directionality on otherwise bidirectional promoters. |
| first_indexed | 2024-03-07T03:44:04Z |
| format | Journal article |
| id | oxford-uuid:bedb025f-a743-41e1-9f59-5b9f4acdcb83 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:44:04Z |
| publishDate | 2012 |
| publisher | American Association for the Advancement of Science |
| record_format | dspace |
| spelling | oxford-uuid:bedb025f-a743-41e1-9f59-5b9f4acdcb832022-03-27T05:43:04ZGene loops enhance transcriptional directionalityJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bedb025f-a743-41e1-9f59-5b9f4acdcb83EnglishSymplectic Elements at OxfordAmerican Association for the Advancement of Science2012Tan-Wong, SZaugg, JCamblong, JXu, ZZhang, DMischo, HAnsari, ALuscombe, NSteinmetz, LProudfoot, NEukaryotic genomes are extensively transcribed, forming both messenger RNAs (mRNAs) and noncoding RNAs (ncRNAs). ncRNAs made by RNA polymerase II often initiate from bidirectional promoters (nucleosome-depleted chromatin) that synthesize mRNA and ncRNA in opposite directions. We demonstrate that, by adopting a gene-loop conformation, actively transcribed mRNA encoding genes restrict divergent transcription of ncRNAs. Because gene-loop formation depends on a protein factor (Ssu72) that coassociates with both the promoter and the terminator, the inactivation of Ssu72 leads to increased synthesis of promoter-associated divergent ncRNAs, referred to as Ssu72-restricted transcripts (SRTs). Similarly, inactivation of individual gene loops by gene mutation enhances SRT synthesis. We demonstrate that gene-loop conformation enforces transcriptional directionality on otherwise bidirectional promoters. |
| spellingShingle | Tan-Wong, S Zaugg, J Camblong, J Xu, Z Zhang, D Mischo, H Ansari, A Luscombe, N Steinmetz, L Proudfoot, N Gene loops enhance transcriptional directionality |
| title | Gene loops enhance transcriptional directionality |
| title_full | Gene loops enhance transcriptional directionality |
| title_fullStr | Gene loops enhance transcriptional directionality |
| title_full_unstemmed | Gene loops enhance transcriptional directionality |
| title_short | Gene loops enhance transcriptional directionality |
| title_sort | gene loops enhance transcriptional directionality |
| work_keys_str_mv | AT tanwongs geneloopsenhancetranscriptionaldirectionality AT zauggj geneloopsenhancetranscriptionaldirectionality AT camblongj geneloopsenhancetranscriptionaldirectionality AT xuz geneloopsenhancetranscriptionaldirectionality AT zhangd geneloopsenhancetranscriptionaldirectionality AT mischoh geneloopsenhancetranscriptionaldirectionality AT ansaria geneloopsenhancetranscriptionaldirectionality AT luscomben geneloopsenhancetranscriptionaldirectionality AT steinmetzl geneloopsenhancetranscriptionaldirectionality AT proudfootn geneloopsenhancetranscriptionaldirectionality |