GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation
Strategies aimed at mimicking or enhancing the action of the incretin hormone glucagon-like peptide 1 (GLP-1) therapeutically improve glucose-stimulated insulin secretion (GSIS); however, it is not clear whether GLP-1 directly drives insulin secretion in pancreatic islets. Here, we examined the mech...
Главные авторы: | , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Формат: | Journal article |
Язык: | English |
Опубликовано: |
American Society for Clinical Investigation
2015
|
_version_ | 1826294389289254912 |
---|---|
author | Zaccolo, M Shigeto, M Ramracheya, R Tarasov, A Cha, C Chibalina, M Hastoy, B Philippaert, K Reinbothe, T Rorsman, N Salehi, A Sones, W Vergari, E Weston, C Gorelik, J Katsura, M Nikolaev, V Vennekens, R Galione, A Johnson, P Kaku, K Ladds, G Rorsman, P |
author_facet | Zaccolo, M Shigeto, M Ramracheya, R Tarasov, A Cha, C Chibalina, M Hastoy, B Philippaert, K Reinbothe, T Rorsman, N Salehi, A Sones, W Vergari, E Weston, C Gorelik, J Katsura, M Nikolaev, V Vennekens, R Galione, A Johnson, P Kaku, K Ladds, G Rorsman, P |
author_sort | Zaccolo, M |
collection | OXFORD |
description | Strategies aimed at mimicking or enhancing the action of the incretin hormone glucagon-like peptide 1 (GLP-1) therapeutically improve glucose-stimulated insulin secretion (GSIS); however, it is not clear whether GLP-1 directly drives insulin secretion in pancreatic islets. Here, we examined the mechanisms by which GLP-1 stimulates insulin secretion in mouse and human islets. We found that GLP-1 enhances GSIS at a half-maximal effective concentration of 0.4 pM. Moreover, we determined that GLP-1 activates PLC, which increases submembrane diacylglycerol and thereby activates PKC, resulting in membrane depolarization and increased action potential firing and subsequent stimulation of insulin secretion. The depolarizing effect of GLP-1 on electrical activity was mimicked by the PKC activator PMA, occurred without activation of PKA, and persisted in the presence of PKA inhibitors, the KATP channel blocker tolbutamide, and the L-type Ca(2+) channel blocker isradipine; however, depolarization was abolished by lowering extracellular Na(+). The PKC-dependent effect of GLP-1 on membrane potential and electrical activity was mediated by activation of Na(+)-permeable TRPM4 and TRPM5 channels by mobilization of intracellular Ca(2+) from thapsigargin-sensitive Ca(2+) stores. Concordantly, GLP-1 effects were negligible in Trpm4 or Trpm5 KO islets. These data provide important insight into the therapeutic action of GLP-1 and suggest that circulating levels of this hormone directly stimulate insulin secretion by β cells. |
first_indexed | 2024-03-07T03:44:52Z |
format | Journal article |
id | oxford-uuid:bf20aef5-debd-4768-9a1e-76c25351ea79 |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-07T03:44:52Z |
publishDate | 2015 |
publisher | American Society for Clinical Investigation |
record_format | dspace |
spelling | oxford-uuid:bf20aef5-debd-4768-9a1e-76c25351ea792022-03-27T05:45:09ZGLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bf20aef5-debd-4768-9a1e-76c25351ea79EnglishSymplectic Elements at OxfordAmerican Society for Clinical Investigation2015Zaccolo, MShigeto, MRamracheya, RTarasov, ACha, CChibalina, MHastoy, BPhilippaert, KReinbothe, TRorsman, NSalehi, ASones, WVergari, EWeston, CGorelik, JKatsura, MNikolaev, VVennekens, RGalione, AJohnson, PKaku, KLadds, GRorsman, PStrategies aimed at mimicking or enhancing the action of the incretin hormone glucagon-like peptide 1 (GLP-1) therapeutically improve glucose-stimulated insulin secretion (GSIS); however, it is not clear whether GLP-1 directly drives insulin secretion in pancreatic islets. Here, we examined the mechanisms by which GLP-1 stimulates insulin secretion in mouse and human islets. We found that GLP-1 enhances GSIS at a half-maximal effective concentration of 0.4 pM. Moreover, we determined that GLP-1 activates PLC, which increases submembrane diacylglycerol and thereby activates PKC, resulting in membrane depolarization and increased action potential firing and subsequent stimulation of insulin secretion. The depolarizing effect of GLP-1 on electrical activity was mimicked by the PKC activator PMA, occurred without activation of PKA, and persisted in the presence of PKA inhibitors, the KATP channel blocker tolbutamide, and the L-type Ca(2+) channel blocker isradipine; however, depolarization was abolished by lowering extracellular Na(+). The PKC-dependent effect of GLP-1 on membrane potential and electrical activity was mediated by activation of Na(+)-permeable TRPM4 and TRPM5 channels by mobilization of intracellular Ca(2+) from thapsigargin-sensitive Ca(2+) stores. Concordantly, GLP-1 effects were negligible in Trpm4 or Trpm5 KO islets. These data provide important insight into the therapeutic action of GLP-1 and suggest that circulating levels of this hormone directly stimulate insulin secretion by β cells. |
spellingShingle | Zaccolo, M Shigeto, M Ramracheya, R Tarasov, A Cha, C Chibalina, M Hastoy, B Philippaert, K Reinbothe, T Rorsman, N Salehi, A Sones, W Vergari, E Weston, C Gorelik, J Katsura, M Nikolaev, V Vennekens, R Galione, A Johnson, P Kaku, K Ladds, G Rorsman, P GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation |
title | GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation |
title_full | GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation |
title_fullStr | GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation |
title_full_unstemmed | GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation |
title_short | GLP-1 stimulates insulin secretion by PKC-dependent TRPM4 and TRPM5 activation |
title_sort | glp 1 stimulates insulin secretion by pkc dependent trpm4 and trpm5 activation |
work_keys_str_mv | AT zaccolom glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT shigetom glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT ramracheyar glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT tarasova glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT chac glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT chibalinam glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT hastoyb glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT philippaertk glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT reinbothet glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT rorsmann glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT salehia glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT sonesw glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT vergarie glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT westonc glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT gorelikj glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT katsuram glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT nikolaevv glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT vennekensr glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT galionea glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT johnsonp glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT kakuk glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT laddsg glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation AT rorsmanp glp1stimulatesinsulinsecretionbypkcdependenttrpm4andtrpm5activation |