A genetically-targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations
Intracellular chloride and pH play fundamental roles in determining a neuron’s synaptic inhibition and excitability. Yet it has been difficult to measure changes in these ions during periods of heightened network activity, such as occur in epilepsy. Here we develop a version of the fluorescent repor...
| Main Authors: | , , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado: |
Cell Press
2023
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| _version_ | 1826310234982842368 |
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| author | Călin, A Waseem, T Raimondo, JV Newey, SE Akerman, CJ |
| author_facet | Călin, A Waseem, T Raimondo, JV Newey, SE Akerman, CJ |
| author_sort | Călin, A |
| collection | OXFORD |
| description | Intracellular chloride and pH play fundamental roles in determining a neuron’s synaptic inhibition and excitability. Yet it has been difficult to measure changes in these ions during periods of heightened network activity, such as occur in epilepsy. Here we develop a version of the fluorescent reporter, ClopHensorN, to enable simultaneous quantification of chloride and pH in genetically defined neurons during epileptiform activity. We compare pyramidal neurons to the major GABAergic interneuron subtypes in mouse hippocampus, which express parvalbumin (PV), somatostatin (SST), or vasoactive intestinal polypeptide (VIP). Interneuron populations exhibit higher baseline chloride, with PV interneurons exhibiting the highest levels. During an epileptiform discharge however, all subtypes converge upon a common elevated chloride level. Concurrent with these dynamics, epileptiform activity leads to different degrees of intracellular acidification, which reflect baseline pH. Thus, a new optical tool for dissociating chloride and pH reveals neuron-specific ion dynamics during heightened network activity. |
| first_indexed | 2024-03-07T07:47:27Z |
| format | Journal article |
| id | oxford-uuid:bfc5b251-94ca-4260-adcb-1fe9410833be |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:47:27Z |
| publishDate | 2023 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:bfc5b251-94ca-4260-adcb-1fe9410833be2023-06-16T11:34:17ZA genetically-targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populationsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bfc5b251-94ca-4260-adcb-1fe9410833beEnglishSymplectic ElementsCell Press2023Călin, AWaseem, TRaimondo, JVNewey, SEAkerman, CJIntracellular chloride and pH play fundamental roles in determining a neuron’s synaptic inhibition and excitability. Yet it has been difficult to measure changes in these ions during periods of heightened network activity, such as occur in epilepsy. Here we develop a version of the fluorescent reporter, ClopHensorN, to enable simultaneous quantification of chloride and pH in genetically defined neurons during epileptiform activity. We compare pyramidal neurons to the major GABAergic interneuron subtypes in mouse hippocampus, which express parvalbumin (PV), somatostatin (SST), or vasoactive intestinal polypeptide (VIP). Interneuron populations exhibit higher baseline chloride, with PV interneurons exhibiting the highest levels. During an epileptiform discharge however, all subtypes converge upon a common elevated chloride level. Concurrent with these dynamics, epileptiform activity leads to different degrees of intracellular acidification, which reflect baseline pH. Thus, a new optical tool for dissociating chloride and pH reveals neuron-specific ion dynamics during heightened network activity. |
| spellingShingle | Călin, A Waseem, T Raimondo, JV Newey, SE Akerman, CJ A genetically-targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations |
| title | A genetically-targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations |
| title_full | A genetically-targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations |
| title_fullStr | A genetically-targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations |
| title_full_unstemmed | A genetically-targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations |
| title_short | A genetically-targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations |
| title_sort | genetically targeted ion sensor reveals distinct seizure related chloride and ph dynamics in gabaergic interneuron populations |
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