Jet-printing microfluidic devices on demand
There is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged microjet...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Wiley
2020
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| _version_ | 1797092508627369984 |
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| author | Soitu, C Stovall-Kurtz, N Deroy, C Castrejon Pita, AA Cook, PR Walsh, EJ |
| author_facet | Soitu, C Stovall-Kurtz, N Deroy, C Castrejon Pita, AA Cook, PR Walsh, EJ |
| author_sort | Soitu, C |
| collection | OXFORD |
| description | There is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged microjet of FC40 is projected through FC40 and media onto the bottom of a dish, where it washes media away to leave liquid fluorocarbon walls pinned to the substrate by interfacial forces. Such fluid walls can be built into almost any imaginable 2D circuit in minutes, which is exploited to clone cells in a way that beats the Poisson limit, subculture adherent cells, and feed arrays of cells continuously for a week. This general method should have wide application in biomedicine. |
| first_indexed | 2024-03-07T03:46:55Z |
| format | Journal article |
| id | oxford-uuid:bfcdfe2e-085a-49ee-9c61-4ab0396d0bbb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:46:55Z |
| publishDate | 2020 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:bfcdfe2e-085a-49ee-9c61-4ab0396d0bbb2022-03-27T05:50:12ZJet-printing microfluidic devices on demandJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:bfcdfe2e-085a-49ee-9c61-4ab0396d0bbbEnglishSymplectic ElementsWiley2020Soitu, CStovall-Kurtz, NDeroy, CCastrejon Pita, AACook, PRWalsh, EJThere is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged microjet of FC40 is projected through FC40 and media onto the bottom of a dish, where it washes media away to leave liquid fluorocarbon walls pinned to the substrate by interfacial forces. Such fluid walls can be built into almost any imaginable 2D circuit in minutes, which is exploited to clone cells in a way that beats the Poisson limit, subculture adherent cells, and feed arrays of cells continuously for a week. This general method should have wide application in biomedicine. |
| spellingShingle | Soitu, C Stovall-Kurtz, N Deroy, C Castrejon Pita, AA Cook, PR Walsh, EJ Jet-printing microfluidic devices on demand |
| title | Jet-printing microfluidic devices on demand |
| title_full | Jet-printing microfluidic devices on demand |
| title_fullStr | Jet-printing microfluidic devices on demand |
| title_full_unstemmed | Jet-printing microfluidic devices on demand |
| title_short | Jet-printing microfluidic devices on demand |
| title_sort | jet printing microfluidic devices on demand |
| work_keys_str_mv | AT soituc jetprintingmicrofluidicdevicesondemand AT stovallkurtzn jetprintingmicrofluidicdevicesondemand AT deroyc jetprintingmicrofluidicdevicesondemand AT castrejonpitaaa jetprintingmicrofluidicdevicesondemand AT cookpr jetprintingmicrofluidicdevicesondemand AT walshej jetprintingmicrofluidicdevicesondemand |