The assembly of large BACs by in vivo recombination.
We have developed a method for recombining bacterial artificial chromosomes (BACs) and P1 artificial chromosomes (PACs) containing large genomic DNA fragments into a single vector using the Cre-lox recombination system from bacteriophage P1 in vivo. This overcomes the limitations of in vitro methods...
Main Authors: | Mejía, J, Larin, Z |
---|---|
格式: | Journal article |
语言: | English |
出版: |
2000
|
相似书籍
-
Recombining overlapping BACs into a single larger BAC
由: Huxley Clare, et al.
出版: (2004-01-01) -
YACs, BACs, PACs and MACs: artificial chromosomes as research tools.
由: Monaco, A, et al.
出版: (1994) -
Construction of recombinant bacmids with immunogenic genes of FMD virus using the Bac-to-Bac system
由: Paulina Andrade, et al.
出版: (2013-04-01) -
Genomic modification of eukaryotes using BAC recombineering
由: A. O. Tsyrulnyk, et al.
出版: (2009-12-01) -
Expression of Functional Recombinant Human Tissue
Transglutaminase (TG2) Using the Bac-to-Bac Baculovirus Expression System
由: Yaghoub Yazdani, et al.
出版: (2016-03-01)