Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2
Hypoxia is central to both ischaemic and neoplastic diseases. However, the non-coding transcriptional response to hypoxia is largely uncharacterized. We undertook integrated genomic analyses of both non-coding and coding transcripts using massively parallel sequencing and interfaced this data with p...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
EMBO Press
2013
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| _version_ | 1797092557417611264 |
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| author | Choudhry, H Schödel, J Oikonomopoulos, S Camps, C Grampp, S Harris, AL Ratcliffe, PJ Ragoussis, J Mole, DR |
| author_facet | Choudhry, H Schödel, J Oikonomopoulos, S Camps, C Grampp, S Harris, AL Ratcliffe, PJ Ragoussis, J Mole, DR |
| author_sort | Choudhry, H |
| collection | OXFORD |
| description | Hypoxia is central to both ischaemic and neoplastic diseases. However, the non-coding transcriptional response to hypoxia is largely uncharacterized. We undertook integrated genomic analyses of both non-coding and coding transcripts using massively parallel sequencing and interfaced this data with pan-genomic analyses of hypoxia-inducible factor (HIF) and RNApol2 binding in hypoxic cells. These analyses revealed that all classes of RNA are profoundly regulated by hypoxia and implicated HIF as a major direct regulator of both the non-coding and coding transcriptome, acting predominantly through release of pre-bound promoter-paused RNApol2. These findings indicate that the transcriptional response to hypoxia is substantially more extensive than previously considered. |
| first_indexed | 2024-03-07T03:47:39Z |
| format | Journal article |
| id | oxford-uuid:c00fed7e-d5c2-4f67-9970-5cfe815fb454 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:47:39Z |
| publishDate | 2013 |
| publisher | EMBO Press |
| record_format | dspace |
| spelling | oxford-uuid:c00fed7e-d5c2-4f67-9970-5cfe815fb4542022-03-27T05:52:05ZExtensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c00fed7e-d5c2-4f67-9970-5cfe815fb454EnglishSymplectic ElementsEMBO Press2013Choudhry, HSchödel, JOikonomopoulos, SCamps, CGrampp, SHarris, ALRatcliffe, PJRagoussis, JMole, DRHypoxia is central to both ischaemic and neoplastic diseases. However, the non-coding transcriptional response to hypoxia is largely uncharacterized. We undertook integrated genomic analyses of both non-coding and coding transcripts using massively parallel sequencing and interfaced this data with pan-genomic analyses of hypoxia-inducible factor (HIF) and RNApol2 binding in hypoxic cells. These analyses revealed that all classes of RNA are profoundly regulated by hypoxia and implicated HIF as a major direct regulator of both the non-coding and coding transcriptome, acting predominantly through release of pre-bound promoter-paused RNApol2. These findings indicate that the transcriptional response to hypoxia is substantially more extensive than previously considered. |
| spellingShingle | Choudhry, H Schödel, J Oikonomopoulos, S Camps, C Grampp, S Harris, AL Ratcliffe, PJ Ragoussis, J Mole, DR Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2 |
| title | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2 |
| title_full | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2 |
| title_fullStr | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2 |
| title_full_unstemmed | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2 |
| title_short | Extensive regulation of the non-coding transcriptome by hypoxia: role of HIF in releasing paused RNApol2 |
| title_sort | extensive regulation of the non coding transcriptome by hypoxia role of hif in releasing paused rnapol2 |
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