An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam.
A procedure for the crystallization of recombinant cytochrome P450cam has been developed which avoids the difficulties inherent in the glass-capillary free-interface diffusion method reported previously. The surface mutation, Cys334-->Ala (C334A), originally designed to prevent dimer formatio...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1998
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| _version_ | 1826294573824999424 |
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| author | Nickerson, D Wong, L Rao, Z |
| author_facet | Nickerson, D Wong, L Rao, Z |
| author_sort | Nickerson, D |
| collection | OXFORD |
| description | A procedure for the crystallization of recombinant cytochrome P450cam has been developed which avoids the difficulties inherent in the glass-capillary free-interface diffusion method reported previously. The surface mutation, Cys334-->Ala (C334A), originally designed to prevent dimer formation and thus improve routine handling of the enzyme, facilitates crystallization by the hanging-drop vapour-diffusion technique. Crystals of (C334A)P450cam grow within 48 h and diffract to beyond 1.2 A at 100 K in-house on a Siemens multiwire area detector. Data have been collected from the camphor-bound form to 1.35 A. |
| first_indexed | 2024-03-07T03:47:45Z |
| format | Journal article |
| id | oxford-uuid:c01b4177-3c4d-4722-ab67-78bdc536d417 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:47:45Z |
| publishDate | 1998 |
| record_format | dspace |
| spelling | oxford-uuid:c01b4177-3c4d-4722-ab67-78bdc536d4172022-03-27T05:52:11ZAn improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c01b4177-3c4d-4722-ab67-78bdc536d417EnglishSymplectic Elements at Oxford1998Nickerson, DWong, LRao, ZA procedure for the crystallization of recombinant cytochrome P450cam has been developed which avoids the difficulties inherent in the glass-capillary free-interface diffusion method reported previously. The surface mutation, Cys334-->Ala (C334A), originally designed to prevent dimer formation and thus improve routine handling of the enzyme, facilitates crystallization by the hanging-drop vapour-diffusion technique. Crystals of (C334A)P450cam grow within 48 h and diffract to beyond 1.2 A at 100 K in-house on a Siemens multiwire area detector. Data have been collected from the camphor-bound form to 1.35 A. |
| spellingShingle | Nickerson, D Wong, L Rao, Z An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam. |
| title | An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam. |
| title_full | An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam. |
| title_fullStr | An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam. |
| title_full_unstemmed | An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam. |
| title_short | An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam. |
| title_sort | improved procedure for the preparation of x ray diffraction quality crystals of cytochrome p450cam |
| work_keys_str_mv | AT nickersond animprovedprocedureforthepreparationofxraydiffractionqualitycrystalsofcytochromep450cam AT wongl animprovedprocedureforthepreparationofxraydiffractionqualitycrystalsofcytochromep450cam AT raoz animprovedprocedureforthepreparationofxraydiffractionqualitycrystalsofcytochromep450cam AT nickersond improvedprocedureforthepreparationofxraydiffractionqualitycrystalsofcytochromep450cam AT wongl improvedprocedureforthepreparationofxraydiffractionqualitycrystalsofcytochromep450cam AT raoz improvedprocedureforthepreparationofxraydiffractionqualitycrystalsofcytochromep450cam |