An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam.

A procedure for the crystallization of recombinant cytochrome P450cam has been developed which avoids the difficulties inherent in the glass-capillary free-interface diffusion method reported previously. The surface mutation, Cys334-->Ala (C334A), originally designed to prevent dimer formatio...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser: Nickerson, D, Wong, L, Rao, Z
Format: Journal article
Sprache:English
Veröffentlicht: 1998