An improved procedure for the preparation of X-ray diffraction-quality crystals of cytochrome p450cam.

A procedure for the crystallization of recombinant cytochrome P450cam has been developed which avoids the difficulties inherent in the glass-capillary free-interface diffusion method reported previously. The surface mutation, Cys334-->Ala (C334A), originally designed to prevent dimer formatio...

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Autori principali: Nickerson, D, Wong, L, Rao, Z
Natura: Journal article
Lingua:English
Pubblicazione: 1998