Structure-guided identification of a non-human morbillivirus with zoonotic potential
Morbilliviruses infect a broad range of mammalian hosts including ruminants, carnivores and humans. The recent eradication of rinderpest virus (RPV), as well as active campaigns for the human-specific measles virus (MeV), have raised significant concerns that the remaining morbilliviruses may emerge...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
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American Society for Microbiology
2018
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| _version_ | 1797092664799133696 |
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| author | Abdullah, N Kelly, J Graham, S Birch, J Gonçalves-Carneiro, D Mitchell, T Thompson, R Lythgoe, K Logan, N Hosie, M Bavro, V Willett, B Heaton, M Bailey, D |
| author_facet | Abdullah, N Kelly, J Graham, S Birch, J Gonçalves-Carneiro, D Mitchell, T Thompson, R Lythgoe, K Logan, N Hosie, M Bavro, V Willett, B Heaton, M Bailey, D |
| author_sort | Abdullah, N |
| collection | OXFORD |
| description | Morbilliviruses infect a broad range of mammalian hosts including ruminants, carnivores and humans. The recent eradication of rinderpest virus (RPV), as well as active campaigns for the human-specific measles virus (MeV), have raised significant concerns that the remaining morbilliviruses may emerge in so-called vacated ecological niches. Seeking to assess the zoonotic-potential of non-human morbilliviruses within human populations we identified that peste des petits ruminants virus (PPRV) - the small ruminant morbillivirus - is restricted at the point of entry into human cells due to deficient interactions with human SLAMF1 – the immune cell receptor for morbilliviruses. Using a structure-guided approach, we characterised a single amino acid change, mapping to the receptor-binding domain in the PPRV Haemagglutinin (H) protein, which overcomes this restriction. The same mutation allowed escape from some cross-protective, human-patient, anti-MeV antibodies, raising concerns that PPRV is a pathogen with zoonotic potential. Analysis of natural variation within human and ovine SLAMF1 also identified polymorphisms that could correlate with disease resistance. Finally, the mechanistic nature of the PPRV restriction was also investigated, identifying charge incompatibility and steric hindrance between PPRV H and human SLAMF1 proteins. Importantly, this research was performed entirely using surrogate virus entry assays, negating the requirement for in situ derivation of a human-tropic PPRV and illustrating alternative strategies for identifying gain-of-function mutations in viral pathogens. |
| first_indexed | 2024-03-07T03:49:15Z |
| format | Journal article |
| id | oxford-uuid:c0a5c30c-aeef-4f87-b165-5fd9d9ff97b0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:49:15Z |
| publishDate | 2018 |
| publisher | American Society for Microbiology |
| record_format | dspace |
| spelling | oxford-uuid:c0a5c30c-aeef-4f87-b165-5fd9d9ff97b02022-03-27T05:55:56ZStructure-guided identification of a non-human morbillivirus with zoonotic potentialJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c0a5c30c-aeef-4f87-b165-5fd9d9ff97b0EnglishSymplectic Elements at OxfordAmerican Society for Microbiology2018Abdullah, NKelly, JGraham, SBirch, JGonçalves-Carneiro, DMitchell, TThompson, RLythgoe, KLogan, NHosie, MBavro, VWillett, BHeaton, MBailey, DMorbilliviruses infect a broad range of mammalian hosts including ruminants, carnivores and humans. The recent eradication of rinderpest virus (RPV), as well as active campaigns for the human-specific measles virus (MeV), have raised significant concerns that the remaining morbilliviruses may emerge in so-called vacated ecological niches. Seeking to assess the zoonotic-potential of non-human morbilliviruses within human populations we identified that peste des petits ruminants virus (PPRV) - the small ruminant morbillivirus - is restricted at the point of entry into human cells due to deficient interactions with human SLAMF1 – the immune cell receptor for morbilliviruses. Using a structure-guided approach, we characterised a single amino acid change, mapping to the receptor-binding domain in the PPRV Haemagglutinin (H) protein, which overcomes this restriction. The same mutation allowed escape from some cross-protective, human-patient, anti-MeV antibodies, raising concerns that PPRV is a pathogen with zoonotic potential. Analysis of natural variation within human and ovine SLAMF1 also identified polymorphisms that could correlate with disease resistance. Finally, the mechanistic nature of the PPRV restriction was also investigated, identifying charge incompatibility and steric hindrance between PPRV H and human SLAMF1 proteins. Importantly, this research was performed entirely using surrogate virus entry assays, negating the requirement for in situ derivation of a human-tropic PPRV and illustrating alternative strategies for identifying gain-of-function mutations in viral pathogens. |
| spellingShingle | Abdullah, N Kelly, J Graham, S Birch, J Gonçalves-Carneiro, D Mitchell, T Thompson, R Lythgoe, K Logan, N Hosie, M Bavro, V Willett, B Heaton, M Bailey, D Structure-guided identification of a non-human morbillivirus with zoonotic potential |
| title | Structure-guided identification of a non-human morbillivirus with zoonotic potential |
| title_full | Structure-guided identification of a non-human morbillivirus with zoonotic potential |
| title_fullStr | Structure-guided identification of a non-human morbillivirus with zoonotic potential |
| title_full_unstemmed | Structure-guided identification of a non-human morbillivirus with zoonotic potential |
| title_short | Structure-guided identification of a non-human morbillivirus with zoonotic potential |
| title_sort | structure guided identification of a non human morbillivirus with zoonotic potential |
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