Several commercially available anti-CCR5 monoclonal antibodies lack specificity and should be used with caution.
CCR5 (CD195) is a receptor for the chemokines RANTES, MIP-1α, and MIP-1β and is used by HIV-1 as a co-receptor for entry into macrophages and CD4+ T cells. CCR5 exists in multiple conformations in the membrane and is present at low levels on human macrophages, making it difficult to detect. Nine com...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1797092709302796288 |
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| author | Bernstone, L van Wilgenburg, B James, W |
| author_facet | Bernstone, L van Wilgenburg, B James, W |
| author_sort | Bernstone, L |
| collection | OXFORD |
| description | CCR5 (CD195) is a receptor for the chemokines RANTES, MIP-1α, and MIP-1β and is used by HIV-1 as a co-receptor for entry into macrophages and CD4+ T cells. CCR5 exists in multiple conformations in the membrane and is present at low levels on human macrophages, making it difficult to detect. Nine commercially available anti-CCR5 monoclonal antibodies were evaluated for their specificity and their recognition of CCR5 expressed by macrophages. Unexpectedly, we found that three of the nine clones tested displayed substantial background binding to CCR5 negative cells, suggesting that these antibodies may give unreliable results. We recommend the use of clones CTC8, 45531 (PE conjugated), and 45523 and advise that data obtained using CTC5, 45531 (unconjugated), and 45549 anti-CCR5 antibody clones should be interpreted with care. |
| first_indexed | 2024-03-07T03:49:52Z |
| format | Journal article |
| id | oxford-uuid:c0dbeac7-f585-4063-aa49-b5dff1697361 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:49:52Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:c0dbeac7-f585-4063-aa49-b5dff16973612022-03-27T05:57:22ZSeveral commercially available anti-CCR5 monoclonal antibodies lack specificity and should be used with caution.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c0dbeac7-f585-4063-aa49-b5dff1697361EnglishSymplectic Elements at Oxford2012Bernstone, Lvan Wilgenburg, BJames, WCCR5 (CD195) is a receptor for the chemokines RANTES, MIP-1α, and MIP-1β and is used by HIV-1 as a co-receptor for entry into macrophages and CD4+ T cells. CCR5 exists in multiple conformations in the membrane and is present at low levels on human macrophages, making it difficult to detect. Nine commercially available anti-CCR5 monoclonal antibodies were evaluated for their specificity and their recognition of CCR5 expressed by macrophages. Unexpectedly, we found that three of the nine clones tested displayed substantial background binding to CCR5 negative cells, suggesting that these antibodies may give unreliable results. We recommend the use of clones CTC8, 45531 (PE conjugated), and 45523 and advise that data obtained using CTC5, 45531 (unconjugated), and 45549 anti-CCR5 antibody clones should be interpreted with care. |
| spellingShingle | Bernstone, L van Wilgenburg, B James, W Several commercially available anti-CCR5 monoclonal antibodies lack specificity and should be used with caution. |
| title | Several commercially available anti-CCR5 monoclonal antibodies lack specificity and should be used with caution. |
| title_full | Several commercially available anti-CCR5 monoclonal antibodies lack specificity and should be used with caution. |
| title_fullStr | Several commercially available anti-CCR5 monoclonal antibodies lack specificity and should be used with caution. |
| title_full_unstemmed | Several commercially available anti-CCR5 monoclonal antibodies lack specificity and should be used with caution. |
| title_short | Several commercially available anti-CCR5 monoclonal antibodies lack specificity and should be used with caution. |
| title_sort | several commercially available anti ccr5 monoclonal antibodies lack specificity and should be used with caution |
| work_keys_str_mv | AT bernstonel severalcommerciallyavailableanticcr5monoclonalantibodieslackspecificityandshouldbeusedwithcaution AT vanwilgenburgb severalcommerciallyavailableanticcr5monoclonalantibodieslackspecificityandshouldbeusedwithcaution AT jamesw severalcommerciallyavailableanticcr5monoclonalantibodieslackspecificityandshouldbeusedwithcaution |