Investigation by photochemically-induced dynamic nuclear polarization and nuclear Overhauser enhancement 1H-NMR of the interaction between beta-endorphin and phospholipid micelles.
The photochemically-induced dynamic nuclear polarization technique has been used to investigate the access of a photoexcited flavin dye to tyrosyl and histidyl residues in [Met]enkephalin and human and camel beta-endorphins, both alone and in the presence of n-dodecylphosphorylcholine micelles. The...
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| Format: | Journal article |
| Language: | English |
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1983
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| _version_ | 1826294719700795392 |
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| author | Zetta, L Hore, P Kaptein, R |
| author_facet | Zetta, L Hore, P Kaptein, R |
| author_sort | Zetta, L |
| collection | OXFORD |
| description | The photochemically-induced dynamic nuclear polarization technique has been used to investigate the access of a photoexcited flavin dye to tyrosyl and histidyl residues in [Met]enkephalin and human and camel beta-endorphins, both alone and in the presence of n-dodecylphosphorylcholine micelles. The results indicate that the mode of binding of Tyr-1, but not of residue 27, is similar in the two endorphins and differs from that of Tyr-1 in [Met]enkephalin. In human beta-endorphin, accessibility and mobility of Tyr-27 are strongly reduced in the presence of lipid at physiological pH, whereas in camel beta-endorphin His-27 becomes immobilized only at high pH. Moreover, nuclear Overhauser enhancement experiments suggest a rigidifying influence of the peptide on the polar head groups of the micelles. |
| first_indexed | 2024-03-07T03:50:01Z |
| format | Journal article |
| id | oxford-uuid:c0e75c07-559d-4d85-b3e8-29ed7ae44667 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:50:01Z |
| publishDate | 1983 |
| record_format | dspace |
| spelling | oxford-uuid:c0e75c07-559d-4d85-b3e8-29ed7ae446672022-03-27T05:57:44ZInvestigation by photochemically-induced dynamic nuclear polarization and nuclear Overhauser enhancement 1H-NMR of the interaction between beta-endorphin and phospholipid micelles.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c0e75c07-559d-4d85-b3e8-29ed7ae44667EnglishSymplectic Elements at Oxford1983Zetta, LHore, PKaptein, RThe photochemically-induced dynamic nuclear polarization technique has been used to investigate the access of a photoexcited flavin dye to tyrosyl and histidyl residues in [Met]enkephalin and human and camel beta-endorphins, both alone and in the presence of n-dodecylphosphorylcholine micelles. The results indicate that the mode of binding of Tyr-1, but not of residue 27, is similar in the two endorphins and differs from that of Tyr-1 in [Met]enkephalin. In human beta-endorphin, accessibility and mobility of Tyr-27 are strongly reduced in the presence of lipid at physiological pH, whereas in camel beta-endorphin His-27 becomes immobilized only at high pH. Moreover, nuclear Overhauser enhancement experiments suggest a rigidifying influence of the peptide on the polar head groups of the micelles. |
| spellingShingle | Zetta, L Hore, P Kaptein, R Investigation by photochemically-induced dynamic nuclear polarization and nuclear Overhauser enhancement 1H-NMR of the interaction between beta-endorphin and phospholipid micelles. |
| title | Investigation by photochemically-induced dynamic nuclear polarization and nuclear Overhauser enhancement 1H-NMR of the interaction between beta-endorphin and phospholipid micelles. |
| title_full | Investigation by photochemically-induced dynamic nuclear polarization and nuclear Overhauser enhancement 1H-NMR of the interaction between beta-endorphin and phospholipid micelles. |
| title_fullStr | Investigation by photochemically-induced dynamic nuclear polarization and nuclear Overhauser enhancement 1H-NMR of the interaction between beta-endorphin and phospholipid micelles. |
| title_full_unstemmed | Investigation by photochemically-induced dynamic nuclear polarization and nuclear Overhauser enhancement 1H-NMR of the interaction between beta-endorphin and phospholipid micelles. |
| title_short | Investigation by photochemically-induced dynamic nuclear polarization and nuclear Overhauser enhancement 1H-NMR of the interaction between beta-endorphin and phospholipid micelles. |
| title_sort | investigation by photochemically induced dynamic nuclear polarization and nuclear overhauser enhancement 1h nmr of the interaction between beta endorphin and phospholipid micelles |
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