A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides.
Two Mucor circinelloides structural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl diph...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1797092724325744640 |
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| author | Velayos, A Fuentes-Vicente, M Aguilar-Elena, R Eslava, A Iturriaga, E |
| author_facet | Velayos, A Fuentes-Vicente, M Aguilar-Elena, R Eslava, A Iturriaga, E |
| author_sort | Velayos, A |
| collection | OXFORD |
| description | Two Mucor circinelloides structural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl diphosphate synthases. By functional complementation in Escherichia coli, the isoB gene product was shown to be a solanesyl diphosphate synthase (EC 2.5.1.11), which is the first fungal enzyme reported having this specificity. In addition, a M. circinelloides one-marker-per-chromosome map was completed by contour-clamped homogeneous electric field localisation of isoA, isoB and three other isoprenoid biosynthesis genes to individual chromosomes. |
| first_indexed | 2024-03-07T03:50:05Z |
| format | Journal article |
| id | oxford-uuid:c0eec1a1-6fb7-4a34-b2cb-d6464b4070e6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:50:05Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:c0eec1a1-6fb7-4a34-b2cb-d6464b4070e62022-03-27T05:57:53ZA novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c0eec1a1-6fb7-4a34-b2cb-d6464b4070e6EnglishSymplectic Elements at Oxford2004Velayos, AFuentes-Vicente, MAguilar-Elena, REslava, AIturriaga, ETwo Mucor circinelloides structural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl diphosphate synthases. By functional complementation in Escherichia coli, the isoB gene product was shown to be a solanesyl diphosphate synthase (EC 2.5.1.11), which is the first fungal enzyme reported having this specificity. In addition, a M. circinelloides one-marker-per-chromosome map was completed by contour-clamped homogeneous electric field localisation of isoA, isoB and three other isoprenoid biosynthesis genes to individual chromosomes. |
| spellingShingle | Velayos, A Fuentes-Vicente, M Aguilar-Elena, R Eslava, A Iturriaga, E A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides. |
| title | A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides. |
| title_full | A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides. |
| title_fullStr | A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides. |
| title_full_unstemmed | A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides. |
| title_short | A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides. |
| title_sort | novel fungal prenyl diphosphate synthase in the dimorphic zygomycete mucor circinelloides |
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