Use of combined Shewhart-CUSUM control charts in internal quality control of enzyme-linked immunosorbent assays for the typing of foot and mouth disease virus antigen.
An enzyme-linked immunosorbent assay (ELISA) for the typing of foot and mouth disease virus (FMDV) antigen was employed for the routine laboratory diagnosis of FMD at a regional veterinary laboratory in northern Thailand. An objective procedure was developed to monitor the test performance of the EL...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1994
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| _version_ | 1797092740053336064 |
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| author | Blacksell, S Gleeson, L Lunt, R Chamnanpood, C |
| author_facet | Blacksell, S Gleeson, L Lunt, R Chamnanpood, C |
| author_sort | Blacksell, S |
| collection | OXFORD |
| description | An enzyme-linked immunosorbent assay (ELISA) for the typing of foot and mouth disease virus (FMDV) antigen was employed for the routine laboratory diagnosis of FMD at a regional veterinary laboratory in northern Thailand. An objective procedure was developed to monitor the test performance of the ELISA, using absolute test control limits in a Shewhart-CUSUM (cumulative sum) control chart method. The procedure detected significant data trends and 'beyond control limit' situations for each antigen typing system (types O, A and Asia 1), using an assay variable (gamma i). Retrospective analysis using Shewhart-CUSUM control charts of data from 42 ELISAs demonstrated that control limits were exceeded in two assays for FMDV type A. The Shewhart-CUSUM control chart is a simple and reliable internal quality control method for the detection of significant random and systematic variation in assays. |
| first_indexed | 2024-03-07T03:50:19Z |
| format | Journal article |
| id | oxford-uuid:c1038bfd-4dc6-4bb6-a632-0642a4fd7a5f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:50:19Z |
| publishDate | 1994 |
| record_format | dspace |
| spelling | oxford-uuid:c1038bfd-4dc6-4bb6-a632-0642a4fd7a5f2022-03-27T05:58:27ZUse of combined Shewhart-CUSUM control charts in internal quality control of enzyme-linked immunosorbent assays for the typing of foot and mouth disease virus antigen.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c1038bfd-4dc6-4bb6-a632-0642a4fd7a5fEnglishSymplectic Elements at Oxford1994Blacksell, SGleeson, LLunt, RChamnanpood, CAn enzyme-linked immunosorbent assay (ELISA) for the typing of foot and mouth disease virus (FMDV) antigen was employed for the routine laboratory diagnosis of FMD at a regional veterinary laboratory in northern Thailand. An objective procedure was developed to monitor the test performance of the ELISA, using absolute test control limits in a Shewhart-CUSUM (cumulative sum) control chart method. The procedure detected significant data trends and 'beyond control limit' situations for each antigen typing system (types O, A and Asia 1), using an assay variable (gamma i). Retrospective analysis using Shewhart-CUSUM control charts of data from 42 ELISAs demonstrated that control limits were exceeded in two assays for FMDV type A. The Shewhart-CUSUM control chart is a simple and reliable internal quality control method for the detection of significant random and systematic variation in assays. |
| spellingShingle | Blacksell, S Gleeson, L Lunt, R Chamnanpood, C Use of combined Shewhart-CUSUM control charts in internal quality control of enzyme-linked immunosorbent assays for the typing of foot and mouth disease virus antigen. |
| title | Use of combined Shewhart-CUSUM control charts in internal quality control of enzyme-linked immunosorbent assays for the typing of foot and mouth disease virus antigen. |
| title_full | Use of combined Shewhart-CUSUM control charts in internal quality control of enzyme-linked immunosorbent assays for the typing of foot and mouth disease virus antigen. |
| title_fullStr | Use of combined Shewhart-CUSUM control charts in internal quality control of enzyme-linked immunosorbent assays for the typing of foot and mouth disease virus antigen. |
| title_full_unstemmed | Use of combined Shewhart-CUSUM control charts in internal quality control of enzyme-linked immunosorbent assays for the typing of foot and mouth disease virus antigen. |
| title_short | Use of combined Shewhart-CUSUM control charts in internal quality control of enzyme-linked immunosorbent assays for the typing of foot and mouth disease virus antigen. |
| title_sort | use of combined shewhart cusum control charts in internal quality control of enzyme linked immunosorbent assays for the typing of foot and mouth disease virus antigen |
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