The retinoblastoma binding protein RBP2 is an H3K4 demethylase.
Changes in histone methylation status regulate chromatin structure and DNA-dependent processes such as transcription. Recent studies indicate that, analogous to other histone modifications, histone methylation is reversible. Retinoblastoma binding protein 2 (RBP2), a nuclear protein implicated in th...
| Główni autorzy: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Język: | English |
| Wydane: |
2007
|
| _version_ | 1826294783222480896 |
|---|---|
| author | Klose, R Yan, Q Tothova, Z Yamane, K Erdjument-Bromage, H Tempst, P Gilliland, D Zhang, Y Kaelin, W |
| author_facet | Klose, R Yan, Q Tothova, Z Yamane, K Erdjument-Bromage, H Tempst, P Gilliland, D Zhang, Y Kaelin, W |
| author_sort | Klose, R |
| collection | OXFORD |
| description | Changes in histone methylation status regulate chromatin structure and DNA-dependent processes such as transcription. Recent studies indicate that, analogous to other histone modifications, histone methylation is reversible. Retinoblastoma binding protein 2 (RBP2), a nuclear protein implicated in the regulation of transcription and differentiation by the retinoblastoma tumor suppressor protein, contains a JmjC domain recently defined as a histone demethylase signature motif. Here we report that RBP2 is a demethylase that specifically catalyzes demethylation on H3K4, whose methylation is normally associated with transcriptionally active genes. RBP2-/- mouse cells displayed enhanced transcription of certain cytokine genes, which, in the case of SDF1, was associated with increased H3K4 trimethylation. Furthermore, RBP2 specifically demethylated H3K4 in biochemical and cell-based assays. These studies provide mechanistic insights into transcriptional regulation by RBP2 and provide the first example of a mammalian enzyme capable of erasing trimethylated H3K4. |
| first_indexed | 2024-03-07T03:51:00Z |
| format | Journal article |
| id | oxford-uuid:c13f2c83-32b5-40dc-a1d9-8ebd6e7cb041 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:51:00Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:c13f2c83-32b5-40dc-a1d9-8ebd6e7cb0412022-03-27T06:00:08ZThe retinoblastoma binding protein RBP2 is an H3K4 demethylase.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c13f2c83-32b5-40dc-a1d9-8ebd6e7cb041EnglishSymplectic Elements at Oxford2007Klose, RYan, QTothova, ZYamane, KErdjument-Bromage, HTempst, PGilliland, DZhang, YKaelin, WChanges in histone methylation status regulate chromatin structure and DNA-dependent processes such as transcription. Recent studies indicate that, analogous to other histone modifications, histone methylation is reversible. Retinoblastoma binding protein 2 (RBP2), a nuclear protein implicated in the regulation of transcription and differentiation by the retinoblastoma tumor suppressor protein, contains a JmjC domain recently defined as a histone demethylase signature motif. Here we report that RBP2 is a demethylase that specifically catalyzes demethylation on H3K4, whose methylation is normally associated with transcriptionally active genes. RBP2-/- mouse cells displayed enhanced transcription of certain cytokine genes, which, in the case of SDF1, was associated with increased H3K4 trimethylation. Furthermore, RBP2 specifically demethylated H3K4 in biochemical and cell-based assays. These studies provide mechanistic insights into transcriptional regulation by RBP2 and provide the first example of a mammalian enzyme capable of erasing trimethylated H3K4. |
| spellingShingle | Klose, R Yan, Q Tothova, Z Yamane, K Erdjument-Bromage, H Tempst, P Gilliland, D Zhang, Y Kaelin, W The retinoblastoma binding protein RBP2 is an H3K4 demethylase. |
| title | The retinoblastoma binding protein RBP2 is an H3K4 demethylase. |
| title_full | The retinoblastoma binding protein RBP2 is an H3K4 demethylase. |
| title_fullStr | The retinoblastoma binding protein RBP2 is an H3K4 demethylase. |
| title_full_unstemmed | The retinoblastoma binding protein RBP2 is an H3K4 demethylase. |
| title_short | The retinoblastoma binding protein RBP2 is an H3K4 demethylase. |
| title_sort | retinoblastoma binding protein rbp2 is an h3k4 demethylase |
| work_keys_str_mv | AT kloser theretinoblastomabindingproteinrbp2isanh3k4demethylase AT yanq theretinoblastomabindingproteinrbp2isanh3k4demethylase AT tothovaz theretinoblastomabindingproteinrbp2isanh3k4demethylase AT yamanek theretinoblastomabindingproteinrbp2isanh3k4demethylase AT erdjumentbromageh theretinoblastomabindingproteinrbp2isanh3k4demethylase AT tempstp theretinoblastomabindingproteinrbp2isanh3k4demethylase AT gillilandd theretinoblastomabindingproteinrbp2isanh3k4demethylase AT zhangy theretinoblastomabindingproteinrbp2isanh3k4demethylase AT kaelinw theretinoblastomabindingproteinrbp2isanh3k4demethylase AT kloser retinoblastomabindingproteinrbp2isanh3k4demethylase AT yanq retinoblastomabindingproteinrbp2isanh3k4demethylase AT tothovaz retinoblastomabindingproteinrbp2isanh3k4demethylase AT yamanek retinoblastomabindingproteinrbp2isanh3k4demethylase AT erdjumentbromageh retinoblastomabindingproteinrbp2isanh3k4demethylase AT tempstp retinoblastomabindingproteinrbp2isanh3k4demethylase AT gillilandd retinoblastomabindingproteinrbp2isanh3k4demethylase AT zhangy retinoblastomabindingproteinrbp2isanh3k4demethylase AT kaelinw retinoblastomabindingproteinrbp2isanh3k4demethylase |