Virological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition.
Human immunodeficiency virus type 1 (HIV-1) can disseminate between CD4(+) T cells via diffusion-limited cell-free viral spread or by directed cell-cell transfer using virally induced structures termed virological synapses. Although T-cell virological synapses have been well characterized, it is unc...
| 主要な著者: | , , , , , |
|---|---|
| フォーマット: | Journal article |
| 言語: | English |
| 出版事項: |
2010
|
| _version_ | 1826294808093655040 |
|---|---|
| author | Martin, N Welsch, S Jolly, C Briggs, J Vaux, D Sattentau, Q |
| author_facet | Martin, N Welsch, S Jolly, C Briggs, J Vaux, D Sattentau, Q |
| author_sort | Martin, N |
| collection | OXFORD |
| description | Human immunodeficiency virus type 1 (HIV-1) can disseminate between CD4(+) T cells via diffusion-limited cell-free viral spread or by directed cell-cell transfer using virally induced structures termed virological synapses. Although T-cell virological synapses have been well characterized, it is unclear whether this mode of viral spread is susceptible to inhibition by neutralizing antibodies and entry inhibitors. We show here that both cell-cell and cell-free viral spread are equivalently sensitive to entry inhibition. Fluorescence imaging analysis measuring virological synapse lifetimes and inhibitor time-of-addition studies implied that inhibitors can access preformed virological synapses and interfere with HIV-1 cell-cell infection. This concept was supported by electron tomography that revealed the T-cell virological synapse to be a relatively permeable structure. Virological synapse-mediated HIV-1 spread is thus efficient but is not an immune or entry inhibitor evasion mechanism, a result that is encouraging for vaccine and drug design. |
| first_indexed | 2024-03-07T03:51:22Z |
| format | Journal article |
| id | oxford-uuid:c15fd23b-779c-40c0-9af8-58c0a6d03e79 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:51:22Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:c15fd23b-779c-40c0-9af8-58c0a6d03e792022-03-27T06:01:03ZVirological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c15fd23b-779c-40c0-9af8-58c0a6d03e79EnglishSymplectic Elements at Oxford2010Martin, NWelsch, SJolly, CBriggs, JVaux, DSattentau, QHuman immunodeficiency virus type 1 (HIV-1) can disseminate between CD4(+) T cells via diffusion-limited cell-free viral spread or by directed cell-cell transfer using virally induced structures termed virological synapses. Although T-cell virological synapses have been well characterized, it is unclear whether this mode of viral spread is susceptible to inhibition by neutralizing antibodies and entry inhibitors. We show here that both cell-cell and cell-free viral spread are equivalently sensitive to entry inhibition. Fluorescence imaging analysis measuring virological synapse lifetimes and inhibitor time-of-addition studies implied that inhibitors can access preformed virological synapses and interfere with HIV-1 cell-cell infection. This concept was supported by electron tomography that revealed the T-cell virological synapse to be a relatively permeable structure. Virological synapse-mediated HIV-1 spread is thus efficient but is not an immune or entry inhibitor evasion mechanism, a result that is encouraging for vaccine and drug design. |
| spellingShingle | Martin, N Welsch, S Jolly, C Briggs, J Vaux, D Sattentau, Q Virological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition. |
| title | Virological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition. |
| title_full | Virological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition. |
| title_fullStr | Virological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition. |
| title_full_unstemmed | Virological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition. |
| title_short | Virological synapse-mediated spread of human immunodeficiency virus type 1 between T cells is sensitive to entry inhibition. |
| title_sort | virological synapse mediated spread of human immunodeficiency virus type 1 between t cells is sensitive to entry inhibition |
| work_keys_str_mv | AT martinn virologicalsynapsemediatedspreadofhumanimmunodeficiencyvirustype1betweentcellsissensitivetoentryinhibition AT welschs virologicalsynapsemediatedspreadofhumanimmunodeficiencyvirustype1betweentcellsissensitivetoentryinhibition AT jollyc virologicalsynapsemediatedspreadofhumanimmunodeficiencyvirustype1betweentcellsissensitivetoentryinhibition AT briggsj virologicalsynapsemediatedspreadofhumanimmunodeficiencyvirustype1betweentcellsissensitivetoentryinhibition AT vauxd virologicalsynapsemediatedspreadofhumanimmunodeficiencyvirustype1betweentcellsissensitivetoentryinhibition AT sattentauq virologicalsynapsemediatedspreadofhumanimmunodeficiencyvirustype1betweentcellsissensitivetoentryinhibition |