Stability of plasma glucose during storage.
Plasma glucose concentrations were found to decrease during storage. This phenomenon was not method-dependent; it was demonstrated using both a Beckman Glucose Analyser 2 (based on glucose oxidase) and a hexokinase-based technique on a centrifugal analyser. The loss of glucose was typically of the o...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1990
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| _version_ | 1826294819634282496 |
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| author | Clark, M Humphreys, S Frayn, K |
| author_facet | Clark, M Humphreys, S Frayn, K |
| author_sort | Clark, M |
| collection | OXFORD |
| description | Plasma glucose concentrations were found to decrease during storage. This phenomenon was not method-dependent; it was demonstrated using both a Beckman Glucose Analyser 2 (based on glucose oxidase) and a hexokinase-based technique on a centrifugal analyser. The loss of glucose was typically of the order of 10% in plasma frozen for periods from 24 h to 14 months. Repeated freezing and thawing (weekly for 1 month) did not produce additional loss of glucose compared with aliquots thawed for the first time at 1 month. Fluoride did not reduce the loss of glucose. Glucose was also lost from frozen neutralized perchloric acid extracts of blood (mean +/- SD: 7 +/- 2 per cent over 1 year). We conclude that this variable loss of glucose on storage may complicate the interpretation of metabolic research procedures. |
| first_indexed | 2024-03-07T03:51:33Z |
| format | Journal article |
| id | oxford-uuid:c17330bb-883b-428f-ac0e-b0d3ee2c2536 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:51:33Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | oxford-uuid:c17330bb-883b-428f-ac0e-b0d3ee2c25362022-03-27T06:01:33ZStability of plasma glucose during storage.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c17330bb-883b-428f-ac0e-b0d3ee2c2536EnglishSymplectic Elements at Oxford1990Clark, MHumphreys, SFrayn, KPlasma glucose concentrations were found to decrease during storage. This phenomenon was not method-dependent; it was demonstrated using both a Beckman Glucose Analyser 2 (based on glucose oxidase) and a hexokinase-based technique on a centrifugal analyser. The loss of glucose was typically of the order of 10% in plasma frozen for periods from 24 h to 14 months. Repeated freezing and thawing (weekly for 1 month) did not produce additional loss of glucose compared with aliquots thawed for the first time at 1 month. Fluoride did not reduce the loss of glucose. Glucose was also lost from frozen neutralized perchloric acid extracts of blood (mean +/- SD: 7 +/- 2 per cent over 1 year). We conclude that this variable loss of glucose on storage may complicate the interpretation of metabolic research procedures. |
| spellingShingle | Clark, M Humphreys, S Frayn, K Stability of plasma glucose during storage. |
| title | Stability of plasma glucose during storage. |
| title_full | Stability of plasma glucose during storage. |
| title_fullStr | Stability of plasma glucose during storage. |
| title_full_unstemmed | Stability of plasma glucose during storage. |
| title_short | Stability of plasma glucose during storage. |
| title_sort | stability of plasma glucose during storage |
| work_keys_str_mv | AT clarkm stabilityofplasmaglucoseduringstorage AT humphreyss stabilityofplasmaglucoseduringstorage AT fraynk stabilityofplasmaglucoseduringstorage |