Characterisation of the human embryonic and foetal epicardium during heart development
The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epi...
| Príomhchruthaitheoirí: | , , , |
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| Formáid: | Journal article |
| Teanga: | English |
| Foilsithe / Cruthaithe: |
Company of Biologists
2015
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| _version_ | 1826312181914796032 |
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| author | Risebro, CA Vieira, JM Klotz, L Riley, PR |
| author_facet | Risebro, CA Vieira, JM Klotz, L Riley, PR |
| author_sort | Risebro, CA |
| collection | OXFORD |
| description | The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epicardium development. We reveal that the epicardium begins to envelop the myocardial surface at Carnegie stage (CS) 11 and this process is completed by CS15, earlier than previously inferred from avian studies. Contrary to prevailing dogma, the formed human epicardium is not a simple squamous epithelium and we reveal evidence of more complex structure, including novel spatial differences aligned to the developing chambers. Specifically, the ventricular, but not atrial, epicardium exhibited areas of expanded epithelium, preferential cell alignment and spindle-like morphology. Likewise, we reveal distinct properties ex vivo, such that ventricular cells spontaneously differentiate and lose epicardial identity, whereas atrial-derived cells remained ‘epithelial-like’. These data provide insight into the developing human epicardium that may contribute to our understanding of congenital heart disease and have implications for the development of strategies for endogenous cell-based cardiac repair. |
| first_indexed | 2024-03-07T08:23:41Z |
| format | Journal article |
| id | oxford-uuid:c19ae969-1e13-4f34-87a7-ef27c36ad764 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:23:41Z |
| publishDate | 2015 |
| publisher | Company of Biologists |
| record_format | dspace |
| spelling | oxford-uuid:c19ae969-1e13-4f34-87a7-ef27c36ad7642024-02-06T10:09:09ZCharacterisation of the human embryonic and foetal epicardium during heart developmentJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c19ae969-1e13-4f34-87a7-ef27c36ad764EnglishSymplectic Elements at OxfordCompany of Biologists2015Risebro, CAVieira, JMKlotz, LRiley, PRThe epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epicardium development. We reveal that the epicardium begins to envelop the myocardial surface at Carnegie stage (CS) 11 and this process is completed by CS15, earlier than previously inferred from avian studies. Contrary to prevailing dogma, the formed human epicardium is not a simple squamous epithelium and we reveal evidence of more complex structure, including novel spatial differences aligned to the developing chambers. Specifically, the ventricular, but not atrial, epicardium exhibited areas of expanded epithelium, preferential cell alignment and spindle-like morphology. Likewise, we reveal distinct properties ex vivo, such that ventricular cells spontaneously differentiate and lose epicardial identity, whereas atrial-derived cells remained ‘epithelial-like’. These data provide insight into the developing human epicardium that may contribute to our understanding of congenital heart disease and have implications for the development of strategies for endogenous cell-based cardiac repair. |
| spellingShingle | Risebro, CA Vieira, JM Klotz, L Riley, PR Characterisation of the human embryonic and foetal epicardium during heart development |
| title | Characterisation of the human embryonic and foetal epicardium during heart development |
| title_full | Characterisation of the human embryonic and foetal epicardium during heart development |
| title_fullStr | Characterisation of the human embryonic and foetal epicardium during heart development |
| title_full_unstemmed | Characterisation of the human embryonic and foetal epicardium during heart development |
| title_short | Characterisation of the human embryonic and foetal epicardium during heart development |
| title_sort | characterisation of the human embryonic and foetal epicardium during heart development |
| work_keys_str_mv | AT risebroca characterisationofthehumanembryonicandfoetalepicardiumduringheartdevelopment AT vieirajm characterisationofthehumanembryonicandfoetalepicardiumduringheartdevelopment AT klotzl characterisationofthehumanembryonicandfoetalepicardiumduringheartdevelopment AT rileypr characterisationofthehumanembryonicandfoetalepicardiumduringheartdevelopment |