Background reduction in STED-FCS using a bivortex phase mask

Fluorescence correlation spectroscopy (FCS) is a valuable tool to study the molecular dynamics in living cells. When used together with a super-resolution stimulated emission depletion (STED) microscope, STED-FCS can measure diffusion processes on the nanoscale in living cells. In two-dimensional (2...

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Bibliographic Details
Main Authors: Barbotin, A, Urbančič, I, Galiani, S, Eggeling, C, Booth, M
Format: Journal article
Language:English
Published: American Chemical Society 2020