Differential and developmental expression of beta-tubulins in a higher plant.
By using two-dimensional gel electrophoresis and immunoblotting, we have analyzed the expression of beta-tubulin isotypes in the higher plant, carrot. We report a complex expression of beta-tubulins that is dependent on the developmental stage of the tissues analyzed. Consequently, each tissue exami...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1988
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| _version_ | 1797092931767631872 |
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| author | Hussey, P Lloyd, C Gull, K |
| author_facet | Hussey, P Lloyd, C Gull, K |
| author_sort | Hussey, P |
| collection | OXFORD |
| description | By using two-dimensional gel electrophoresis and immunoblotting, we have analyzed the expression of beta-tubulin isotypes in the higher plant, carrot. We report a complex expression of beta-tubulins that is dependent on the developmental stage of the tissues analyzed. Consequently, each tissue examined can be identified by its unique composition of beta-tubulins. In total, there are six electrophoretically separable beta-tubulins. In no tissue, however, is there less than two or more than five beta-tubulins. Within this framework we have detected a beta-tubulin specific to seedling tissue beta 6, and a beta-tubulin, beta 5, that is found only in the vegetative tissues of the mature plant. Traced from stem to midrib to leaf lamina, the beta 5 isotype becomes progressively dominant relative to beta 1. Another beta-tubulin isotype, beta 4, appears in marked abundance in immature and mature stamens. In isolated mature pollen the beta 4-tubulin overwhelmingly predominates the ubiquitously expressed beta 2-tubulin isotype. The remaining beta-tubulin isotypes also have specific expression programs with beta 1 present in all tissues except pollen and beta 3 absent only from pollen and leafy tissues. |
| first_indexed | 2024-03-07T03:53:06Z |
| format | Journal article |
| id | oxford-uuid:c1f2f9ee-2dee-4a35-9389-dd57ee0d46bb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:53:06Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:c1f2f9ee-2dee-4a35-9389-dd57ee0d46bb2022-03-27T06:05:19ZDifferential and developmental expression of beta-tubulins in a higher plant.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c1f2f9ee-2dee-4a35-9389-dd57ee0d46bbEnglishSymplectic Elements at Oxford1988Hussey, PLloyd, CGull, KBy using two-dimensional gel electrophoresis and immunoblotting, we have analyzed the expression of beta-tubulin isotypes in the higher plant, carrot. We report a complex expression of beta-tubulins that is dependent on the developmental stage of the tissues analyzed. Consequently, each tissue examined can be identified by its unique composition of beta-tubulins. In total, there are six electrophoretically separable beta-tubulins. In no tissue, however, is there less than two or more than five beta-tubulins. Within this framework we have detected a beta-tubulin specific to seedling tissue beta 6, and a beta-tubulin, beta 5, that is found only in the vegetative tissues of the mature plant. Traced from stem to midrib to leaf lamina, the beta 5 isotype becomes progressively dominant relative to beta 1. Another beta-tubulin isotype, beta 4, appears in marked abundance in immature and mature stamens. In isolated mature pollen the beta 4-tubulin overwhelmingly predominates the ubiquitously expressed beta 2-tubulin isotype. The remaining beta-tubulin isotypes also have specific expression programs with beta 1 present in all tissues except pollen and beta 3 absent only from pollen and leafy tissues. |
| spellingShingle | Hussey, P Lloyd, C Gull, K Differential and developmental expression of beta-tubulins in a higher plant. |
| title | Differential and developmental expression of beta-tubulins in a higher plant. |
| title_full | Differential and developmental expression of beta-tubulins in a higher plant. |
| title_fullStr | Differential and developmental expression of beta-tubulins in a higher plant. |
| title_full_unstemmed | Differential and developmental expression of beta-tubulins in a higher plant. |
| title_short | Differential and developmental expression of beta-tubulins in a higher plant. |
| title_sort | differential and developmental expression of beta tubulins in a higher plant |
| work_keys_str_mv | AT husseyp differentialanddevelopmentalexpressionofbetatubulinsinahigherplant AT lloydc differentialanddevelopmentalexpressionofbetatubulinsinahigherplant AT gullk differentialanddevelopmentalexpressionofbetatubulinsinahigherplant |