Assessment of microvessel density and carbonic anhydrase-9 (CA-9) expression in rectal cancer.
AIM: The mechanism by which neoplasias respond to hypoxia determines their biological behavior and prognosis. Understanding the biology of tumors under hypoxic conditions is crucial for the development of anti-angiogenic therapy. Using the largest cohort of rectal adenocarcinomas to date, this stud...
| Päätekijät: | , , , , , , , , , |
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| Aineistotyyppi: | Journal article |
| Kieli: | English |
| Julkaistu: |
2009
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| _version_ | 1826294946061090816 |
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| author | Rasheed, S Harris, A Tekkis, P Turley, H Silver, A McDonald, P Talbot, I Glynne-Jones, R Northover, J Guenther, T |
| author_facet | Rasheed, S Harris, A Tekkis, P Turley, H Silver, A McDonald, P Talbot, I Glynne-Jones, R Northover, J Guenther, T |
| author_sort | Rasheed, S |
| collection | OXFORD |
| description | AIM: The mechanism by which neoplasias respond to hypoxia determines their biological behavior and prognosis. Understanding the biology of tumors under hypoxic conditions is crucial for the development of anti-angiogenic therapy. Using the largest cohort of rectal adenocarcinomas to date, this study aimed to assess microvessel density (MVD) and carbonic anhydrase-9 (CA-9) expression and to correlate the results with recurrence and cancer-specific survival. MATERIALS AND METHODS: Patients (n=101) who underwent surgery for rectal adenocarcinoma without previous neoadjuvant therapy or metastatic disease were selected. MVD and CA-9 expression were assessed immunohistologically by using the CD34 antibody and the MN/CA9 M75 antibody, respectively. In a multifactorial analysis, the results were correlated with tumor stage, recurrence rate, and long-term survival. RESULTS: MVD was higher with increased T- and N-stages (p<0.01) and associated positively with poor survival (hazard ratio (HR) 1.3 per 10 vessel increase, p<0.01). CA-9 was expressed in 73% of cancers. Negative lymph node status correlated with CA-9 positivity (p<0.05), reflected in a higher rate of CA-9 positivity in earlier Dukes' stages (p<0.05). CA-9 positivity across tumor node metastasis (TNM) stages approached significance (Stage I/II: 80% CA-9 positive vs. 20% CA-9 negative; Stage III: 63% CA-9 positive vs. 37% negative, p=0.051). A trend was seen towards better cancer-specific survival in patients with CA-9 positive carcinomas (HR 0.51, p=0.07) on univariate analysis. DISCUSSION: MVD was higher in more advanced T- and N-stages and may be used as a determinant of survival in patients with rectal adenocarcinomas. CA-9 expression was seen more often in earlier Dukes' stages, possibly representing an early tumor hypoxic response. CA-9 expression by adenocarcinoma cells may confer long-term survival advantage in surgically treated rectal cancer. |
| first_indexed | 2024-03-07T03:53:32Z |
| format | Journal article |
| id | oxford-uuid:c2161d5a-7cdb-4a5a-b2a2-2db22fd145d6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:53:32Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:c2161d5a-7cdb-4a5a-b2a2-2db22fd145d62022-03-27T06:06:25ZAssessment of microvessel density and carbonic anhydrase-9 (CA-9) expression in rectal cancer.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c2161d5a-7cdb-4a5a-b2a2-2db22fd145d6EnglishSymplectic Elements at Oxford2009Rasheed, SHarris, ATekkis, PTurley, HSilver, AMcDonald, PTalbot, IGlynne-Jones, RNorthover, JGuenther, T AIM: The mechanism by which neoplasias respond to hypoxia determines their biological behavior and prognosis. Understanding the biology of tumors under hypoxic conditions is crucial for the development of anti-angiogenic therapy. Using the largest cohort of rectal adenocarcinomas to date, this study aimed to assess microvessel density (MVD) and carbonic anhydrase-9 (CA-9) expression and to correlate the results with recurrence and cancer-specific survival. MATERIALS AND METHODS: Patients (n=101) who underwent surgery for rectal adenocarcinoma without previous neoadjuvant therapy or metastatic disease were selected. MVD and CA-9 expression were assessed immunohistologically by using the CD34 antibody and the MN/CA9 M75 antibody, respectively. In a multifactorial analysis, the results were correlated with tumor stage, recurrence rate, and long-term survival. RESULTS: MVD was higher with increased T- and N-stages (p<0.01) and associated positively with poor survival (hazard ratio (HR) 1.3 per 10 vessel increase, p<0.01). CA-9 was expressed in 73% of cancers. Negative lymph node status correlated with CA-9 positivity (p<0.05), reflected in a higher rate of CA-9 positivity in earlier Dukes' stages (p<0.05). CA-9 positivity across tumor node metastasis (TNM) stages approached significance (Stage I/II: 80% CA-9 positive vs. 20% CA-9 negative; Stage III: 63% CA-9 positive vs. 37% negative, p=0.051). A trend was seen towards better cancer-specific survival in patients with CA-9 positive carcinomas (HR 0.51, p=0.07) on univariate analysis. DISCUSSION: MVD was higher in more advanced T- and N-stages and may be used as a determinant of survival in patients with rectal adenocarcinomas. CA-9 expression was seen more often in earlier Dukes' stages, possibly representing an early tumor hypoxic response. CA-9 expression by adenocarcinoma cells may confer long-term survival advantage in surgically treated rectal cancer. |
| spellingShingle | Rasheed, S Harris, A Tekkis, P Turley, H Silver, A McDonald, P Talbot, I Glynne-Jones, R Northover, J Guenther, T Assessment of microvessel density and carbonic anhydrase-9 (CA-9) expression in rectal cancer. |
| title | Assessment of microvessel density and carbonic anhydrase-9 (CA-9) expression in rectal cancer. |
| title_full | Assessment of microvessel density and carbonic anhydrase-9 (CA-9) expression in rectal cancer. |
| title_fullStr | Assessment of microvessel density and carbonic anhydrase-9 (CA-9) expression in rectal cancer. |
| title_full_unstemmed | Assessment of microvessel density and carbonic anhydrase-9 (CA-9) expression in rectal cancer. |
| title_short | Assessment of microvessel density and carbonic anhydrase-9 (CA-9) expression in rectal cancer. |
| title_sort | assessment of microvessel density and carbonic anhydrase 9 ca 9 expression in rectal cancer |
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