Active transport of cytoophidia in Schizosaccharomyces pombe
The metabolic enzyme cytidine triphosphate synthase has recently been found to form micrometer-sized filamentous structures termed cytoophidia, which are evolutionarily conserved across prokaryotes and eukaryotes. The cytoophidium represents a novel type of membraneless organelle and behaves dynamic...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Federation of American Societies for Experimental Biology
2018
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| _version_ | 1826295173407047680 |
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| author | Li, H Ye, F Ren, J-Y Wang, P-Y Du, L-L Liu, J-L |
| author_facet | Li, H Ye, F Ren, J-Y Wang, P-Y Du, L-L Liu, J-L |
| author_sort | Li, H |
| collection | OXFORD |
| description | The metabolic enzyme cytidine triphosphate synthase has recently been found to form micrometer-sized filamentous structures termed cytoophidia, which are evolutionarily conserved across prokaryotes and eukaryotes. The cytoophidium represents a novel type of membraneless organelle and behaves dynamically inside the cell. The question of how cytoophidia transport is mediated, however, remains unanswered. For the first time, we detected in this study the active transport of cytoophidia, taking advantage of the fission yeast Schizosaccharomyces pombe as an excellent model for studying membraneless organelles. We demonstrated that actin filaments, not microtubules, are responsible for this transport. Furthermore, we determined that Myo52, a type of myosin V, is required for the active transport of cytoophidia. These results reveal the major players critical to the dynamics of cytoophidia and extend our understanding of intracellular transport of membraneless organelles.—Li, H., Ye, F., Ren, J.-Y., Wang, P.-Y., Du, L.-L., Liu, J.-L. Active transport of cytoophidia in Schizosaccharomyces pombe. |
| first_indexed | 2024-03-07T03:56:58Z |
| format | Journal article |
| id | oxford-uuid:c333e373-4649-4026-bfd2-a89043256782 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:56:58Z |
| publishDate | 2018 |
| publisher | Federation of American Societies for Experimental Biology |
| record_format | dspace |
| spelling | oxford-uuid:c333e373-4649-4026-bfd2-a890432567822022-03-27T06:14:44ZActive transport of cytoophidia in Schizosaccharomyces pombeJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c333e373-4649-4026-bfd2-a89043256782EnglishSymplectic Elements at OxfordFederation of American Societies for Experimental Biology2018Li, HYe, FRen, J-YWang, P-YDu, L-LLiu, J-LThe metabolic enzyme cytidine triphosphate synthase has recently been found to form micrometer-sized filamentous structures termed cytoophidia, which are evolutionarily conserved across prokaryotes and eukaryotes. The cytoophidium represents a novel type of membraneless organelle and behaves dynamically inside the cell. The question of how cytoophidia transport is mediated, however, remains unanswered. For the first time, we detected in this study the active transport of cytoophidia, taking advantage of the fission yeast Schizosaccharomyces pombe as an excellent model for studying membraneless organelles. We demonstrated that actin filaments, not microtubules, are responsible for this transport. Furthermore, we determined that Myo52, a type of myosin V, is required for the active transport of cytoophidia. These results reveal the major players critical to the dynamics of cytoophidia and extend our understanding of intracellular transport of membraneless organelles.—Li, H., Ye, F., Ren, J.-Y., Wang, P.-Y., Du, L.-L., Liu, J.-L. Active transport of cytoophidia in Schizosaccharomyces pombe. |
| spellingShingle | Li, H Ye, F Ren, J-Y Wang, P-Y Du, L-L Liu, J-L Active transport of cytoophidia in Schizosaccharomyces pombe |
| title | Active transport of cytoophidia in Schizosaccharomyces pombe |
| title_full | Active transport of cytoophidia in Schizosaccharomyces pombe |
| title_fullStr | Active transport of cytoophidia in Schizosaccharomyces pombe |
| title_full_unstemmed | Active transport of cytoophidia in Schizosaccharomyces pombe |
| title_short | Active transport of cytoophidia in Schizosaccharomyces pombe |
| title_sort | active transport of cytoophidia in schizosaccharomyces pombe |
| work_keys_str_mv | AT lih activetransportofcytoophidiainschizosaccharomycespombe AT yef activetransportofcytoophidiainschizosaccharomycespombe AT renjy activetransportofcytoophidiainschizosaccharomycespombe AT wangpy activetransportofcytoophidiainschizosaccharomycespombe AT dull activetransportofcytoophidiainschizosaccharomycespombe AT liujl activetransportofcytoophidiainschizosaccharomycespombe |