Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model
The surfaceome is critical because surface proteins provide a gateway for internal signals and transfer of molecules into cells, and surfaceome differences can influence therapy response. We have used a surfaceome analysis method, based on comparing RNA-seq data between normal and abnormal cells (Su...
| Main Authors: | , , , , , , , , , , |
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| 格式: | Journal article |
| 语言: | English |
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Springer Nature
2019
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| _version_ | 1826295234842066944 |
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| author | Pais, H Ruggero, K Zhang, J Al-Assar, O Bery, N Bhuller, R Weston, V Kearns, P Mecucci, C Miller, A Rabbitts, T |
| author_facet | Pais, H Ruggero, K Zhang, J Al-Assar, O Bery, N Bhuller, R Weston, V Kearns, P Mecucci, C Miller, A Rabbitts, T |
| author_sort | Pais, H |
| collection | OXFORD |
| description | The surfaceome is critical because surface proteins provide a gateway for internal signals and transfer of molecules into cells, and surfaceome differences can influence therapy response. We have used a surfaceome analysis method, based on comparing RNA-seq data between normal and abnormal cells (Surfaceome DataBase Mining or Surfaceome DBM), to identify sets of upregulated cell surface protein mRNAs in an LMO2-mediated T-ALL mouse model and corroborated by protein detection using antibodies. In this model the leukemia initiating cells (LICs) comprise pre-leukaemic, differentiation inhibited thymocytes allowing us to provide a profile of the LIC surfaceome in which GPR56, CD53 and CD59a are co-expressed with CD25. Implementation of cell surface interaction assays demonstrates fluid interaction of surface proteins and CD25 is only internalized when co-localized with other proteins. The Surfaceome DBM approach to analyse cancer cell surfaceomes is a way to find targetable surface biomarkers for clinical conditions where RNA-seq data from normal and abnormal cell are available. |
| first_indexed | 2024-03-07T03:57:54Z |
| format | Journal article |
| id | oxford-uuid:c37f746e-a2ee-40b6-9bb2-84bfd1c2d7f3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:57:54Z |
| publishDate | 2019 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:c37f746e-a2ee-40b6-9bb2-84bfd1c2d7f32022-03-27T06:16:56ZSurfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic modelJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c37f746e-a2ee-40b6-9bb2-84bfd1c2d7f3EnglishSymplectic Elements at OxfordSpringer Nature2019Pais, HRuggero, KZhang, JAl-Assar, OBery, NBhuller, RWeston, VKearns, PMecucci, CMiller, ARabbitts, TThe surfaceome is critical because surface proteins provide a gateway for internal signals and transfer of molecules into cells, and surfaceome differences can influence therapy response. We have used a surfaceome analysis method, based on comparing RNA-seq data between normal and abnormal cells (Surfaceome DataBase Mining or Surfaceome DBM), to identify sets of upregulated cell surface protein mRNAs in an LMO2-mediated T-ALL mouse model and corroborated by protein detection using antibodies. In this model the leukemia initiating cells (LICs) comprise pre-leukaemic, differentiation inhibited thymocytes allowing us to provide a profile of the LIC surfaceome in which GPR56, CD53 and CD59a are co-expressed with CD25. Implementation of cell surface interaction assays demonstrates fluid interaction of surface proteins and CD25 is only internalized when co-localized with other proteins. The Surfaceome DBM approach to analyse cancer cell surfaceomes is a way to find targetable surface biomarkers for clinical conditions where RNA-seq data from normal and abnormal cell are available. |
| spellingShingle | Pais, H Ruggero, K Zhang, J Al-Assar, O Bery, N Bhuller, R Weston, V Kearns, P Mecucci, C Miller, A Rabbitts, T Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model |
| title | Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model |
| title_full | Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model |
| title_fullStr | Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model |
| title_full_unstemmed | Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model |
| title_short | Surfaceome interrogation using an RNA-seq approach highlights leukemia initiating cell biomarkers in an LMO2 T cell transgenic model |
| title_sort | surfaceome interrogation using an rna seq approach highlights leukemia initiating cell biomarkers in an lmo2 t cell transgenic model |
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