Fluorescent PSC-derived cardiomyocyte reporter lines: generation approaches and their applications in cardiovascular medicine
Recent advances have made pluripotent stem cell (PSC)-derived cardiomyocytes an attractive option to model both normal and diseased cardiac function at the single-cell level. However, in vitro differentiation yields heterogeneous populations of cardiomyocytes and other cell types, potentially confou...
| Main Authors: | , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
MDPI
2020
|
| _version_ | 1826295240200290304 |
|---|---|
| author | Sontayananon, N Redwood, C Davies, B Gehmlich, K |
| author_facet | Sontayananon, N Redwood, C Davies, B Gehmlich, K |
| author_sort | Sontayananon, N |
| collection | OXFORD |
| description | Recent advances have made pluripotent stem cell (PSC)-derived cardiomyocytes an attractive option to model both normal and diseased cardiac function at the single-cell level. However, in vitro differentiation yields heterogeneous populations of cardiomyocytes and other cell types, potentially confounding phenotypic analyses. Fluorescent PSC-derived cardiomyocyte reporter systems allow specific cell lineages to be labelled, facilitating cell isolation for downstream applications including drug testing, disease modelling and cardiac regeneration. In this review, the different genetic strategies used to generate such reporter lines are presented with an emphasis on their relative technical advantages and disadvantages. Next, we explore how the fluorescent reporter lines have provided insights into cardiac development and cardiomyocyte physiology. Finally, we discuss how exciting new approaches using PSC-derived cardiomyocyte reporter lines are contributing to progress in cardiac cell therapy with respect to both graft adaptation and clinical safety. |
| first_indexed | 2024-03-07T03:57:59Z |
| format | Journal article |
| id | oxford-uuid:c386cd73-a4ae-4f0b-9c2e-5510386d9ed8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:57:59Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oxford-uuid:c386cd73-a4ae-4f0b-9c2e-5510386d9ed82022-03-27T06:17:07ZFluorescent PSC-derived cardiomyocyte reporter lines: generation approaches and their applications in cardiovascular medicine Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c386cd73-a4ae-4f0b-9c2e-5510386d9ed8EnglishSymplectic ElementsMDPI2020Sontayananon, NRedwood, CDavies, BGehmlich, KRecent advances have made pluripotent stem cell (PSC)-derived cardiomyocytes an attractive option to model both normal and diseased cardiac function at the single-cell level. However, in vitro differentiation yields heterogeneous populations of cardiomyocytes and other cell types, potentially confounding phenotypic analyses. Fluorescent PSC-derived cardiomyocyte reporter systems allow specific cell lineages to be labelled, facilitating cell isolation for downstream applications including drug testing, disease modelling and cardiac regeneration. In this review, the different genetic strategies used to generate such reporter lines are presented with an emphasis on their relative technical advantages and disadvantages. Next, we explore how the fluorescent reporter lines have provided insights into cardiac development and cardiomyocyte physiology. Finally, we discuss how exciting new approaches using PSC-derived cardiomyocyte reporter lines are contributing to progress in cardiac cell therapy with respect to both graft adaptation and clinical safety. |
| spellingShingle | Sontayananon, N Redwood, C Davies, B Gehmlich, K Fluorescent PSC-derived cardiomyocyte reporter lines: generation approaches and their applications in cardiovascular medicine |
| title | Fluorescent PSC-derived cardiomyocyte reporter lines: generation approaches and their applications in cardiovascular medicine |
| title_full | Fluorescent PSC-derived cardiomyocyte reporter lines: generation approaches and their applications in cardiovascular medicine |
| title_fullStr | Fluorescent PSC-derived cardiomyocyte reporter lines: generation approaches and their applications in cardiovascular medicine |
| title_full_unstemmed | Fluorescent PSC-derived cardiomyocyte reporter lines: generation approaches and their applications in cardiovascular medicine |
| title_short | Fluorescent PSC-derived cardiomyocyte reporter lines: generation approaches and their applications in cardiovascular medicine |
| title_sort | fluorescent psc derived cardiomyocyte reporter lines generation approaches and their applications in cardiovascular medicine |
| work_keys_str_mv | AT sontayananonn fluorescentpscderivedcardiomyocytereporterlinesgenerationapproachesandtheirapplicationsincardiovascularmedicine AT redwoodc fluorescentpscderivedcardiomyocytereporterlinesgenerationapproachesandtheirapplicationsincardiovascularmedicine AT daviesb fluorescentpscderivedcardiomyocytereporterlinesgenerationapproachesandtheirapplicationsincardiovascularmedicine AT gehmlichk fluorescentpscderivedcardiomyocytereporterlinesgenerationapproachesandtheirapplicationsincardiovascularmedicine |