The cytotoxic action of lymphokine activated killer cells upon the human glioma cell line U251 is stimulated by bispecific monoclonal antibody (MoAb) constructs.
The ability of IL-2 stimulated mononuclear cells to kill the human glioblastoma cell line U251 has been investigated. Highest cytotoxic activity was generated in low cell density cultures incubated for 15 days with 250-1000 U/ml IL-2. Sub-optimal killing was noted, with cells only exposed to IL-2 fo...
| Main Authors: | , , , , , |
|---|---|
| פורמט: | Journal article |
| שפה: | English |
| יצא לאור: |
1992
|
| _version_ | 1826295272161935360 |
|---|---|
| author | Obukhov, S Gennie, M Tutt, A Kemshead, J Coakham, H Beverely, P |
| author_facet | Obukhov, S Gennie, M Tutt, A Kemshead, J Coakham, H Beverely, P |
| author_sort | Obukhov, S |
| collection | OXFORD |
| description | The ability of IL-2 stimulated mononuclear cells to kill the human glioblastoma cell line U251 has been investigated. Highest cytotoxic activity was generated in low cell density cultures incubated for 15 days with 250-1000 U/ml IL-2. Sub-optimal killing was noted, with cells only exposed to IL-2 for three days. Under the latter conditions, bispecific monoclonal antibodies (MoAbs) of either anti-CD3 or anti-CD16 and an anti-NCAM MoAb stimulated LAK cell activity markedly. Anti-CD16 conjugates were found more effective than anti-CD3 and (Fab')2 constructs more efficacious than those made with whole Ig molecules. Maximal stimulation of LAK cell activity was noted with bispecific MoAbs. Little effect was observed with either single or mixtures of monomeric MoAbs. Furthermore, no effect of bispecific MoAbs was observed when target cells lacked expression of NCAM. These results could be of clinical importance as it is not always feasible to screen LAK cells for optimal activity before administration to patients. Whilst bispecific MoAbs have no effect on optimally stimulated LAK cells, they are not inhibitory and can stimulate killing under sub-optimal IL-2 stimulation. |
| first_indexed | 2024-03-07T03:58:28Z |
| format | Journal article |
| id | oxford-uuid:c3b0614c-653c-4c0f-8d15-f092d50ef9df |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T03:58:28Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:c3b0614c-653c-4c0f-8d15-f092d50ef9df2022-03-27T06:18:18ZThe cytotoxic action of lymphokine activated killer cells upon the human glioma cell line U251 is stimulated by bispecific monoclonal antibody (MoAb) constructs.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c3b0614c-653c-4c0f-8d15-f092d50ef9dfEnglishSymplectic Elements at Oxford1992Obukhov, SGennie, MTutt, AKemshead, JCoakham, HBeverely, PThe ability of IL-2 stimulated mononuclear cells to kill the human glioblastoma cell line U251 has been investigated. Highest cytotoxic activity was generated in low cell density cultures incubated for 15 days with 250-1000 U/ml IL-2. Sub-optimal killing was noted, with cells only exposed to IL-2 for three days. Under the latter conditions, bispecific monoclonal antibodies (MoAbs) of either anti-CD3 or anti-CD16 and an anti-NCAM MoAb stimulated LAK cell activity markedly. Anti-CD16 conjugates were found more effective than anti-CD3 and (Fab')2 constructs more efficacious than those made with whole Ig molecules. Maximal stimulation of LAK cell activity was noted with bispecific MoAbs. Little effect was observed with either single or mixtures of monomeric MoAbs. Furthermore, no effect of bispecific MoAbs was observed when target cells lacked expression of NCAM. These results could be of clinical importance as it is not always feasible to screen LAK cells for optimal activity before administration to patients. Whilst bispecific MoAbs have no effect on optimally stimulated LAK cells, they are not inhibitory and can stimulate killing under sub-optimal IL-2 stimulation. |
| spellingShingle | Obukhov, S Gennie, M Tutt, A Kemshead, J Coakham, H Beverely, P The cytotoxic action of lymphokine activated killer cells upon the human glioma cell line U251 is stimulated by bispecific monoclonal antibody (MoAb) constructs. |
| title | The cytotoxic action of lymphokine activated killer cells upon the human glioma cell line U251 is stimulated by bispecific monoclonal antibody (MoAb) constructs. |
| title_full | The cytotoxic action of lymphokine activated killer cells upon the human glioma cell line U251 is stimulated by bispecific monoclonal antibody (MoAb) constructs. |
| title_fullStr | The cytotoxic action of lymphokine activated killer cells upon the human glioma cell line U251 is stimulated by bispecific monoclonal antibody (MoAb) constructs. |
| title_full_unstemmed | The cytotoxic action of lymphokine activated killer cells upon the human glioma cell line U251 is stimulated by bispecific monoclonal antibody (MoAb) constructs. |
| title_short | The cytotoxic action of lymphokine activated killer cells upon the human glioma cell line U251 is stimulated by bispecific monoclonal antibody (MoAb) constructs. |
| title_sort | cytotoxic action of lymphokine activated killer cells upon the human glioma cell line u251 is stimulated by bispecific monoclonal antibody moab constructs |
| work_keys_str_mv | AT obukhovs thecytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT genniem thecytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT tutta thecytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT kemsheadj thecytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT coakhamh thecytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT beverelyp thecytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT obukhovs cytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT genniem cytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT tutta cytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT kemsheadj cytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT coakhamh cytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs AT beverelyp cytotoxicactionoflymphokineactivatedkillercellsuponthehumangliomacelllineu251isstimulatedbybispecificmonoclonalantibodymoabconstructs |