Simultaneous identification of species and molecular forms of the Anopheles gambiae complex by PCR-RFLP.

For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper tha...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser: Fanello, C, Santolamazza, F, della Torre, A
Format: Journal article
Sprache:English
Veröffentlicht: 2002
Beschreibung
Zusammenfassung:For differential identification of sibling species in the Anopheles gambiae Giles complex (Diptera: Culicidae), including simultaneous separation of M and S molecular forms within An. gambiae Giles sensu stricto, we describe a PCR-RFLP method. This procedure is more efficient, faster and cheaper than those used before, so is recommended for large-scale processing of field-collected larval and adult specimens to be identified in malaria vector studies.