A simple mechanism for the establishment of C2‐specific gene expression in Brassicaceae
<p>The transition of C<sub>3</sub>, via C<sub>2</sub> towards C<sub>4</sub> photosynthesis is an important example of stepwise evolution of a complex genetic trait. A common feature that was gradually emphasized during this trajectory is th...
| Egile Nagusiak: | , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
Wiley
2015
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| _version_ | 1826295387114176512 |
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| author | Adwy, W Laxa, M Peterhansel, C |
| author_facet | Adwy, W Laxa, M Peterhansel, C |
| author_sort | Adwy, W |
| collection | OXFORD |
| description | <p>The transition of C<sub>3</sub>, via C<sub>2</sub> towards C<sub>4</sub> photosynthesis is an important example of stepwise evolution of a complex genetic trait. A common feature that was gradually emphasized during this trajectory is the evolution of a CO<sub>2</sub> concentration mechanism around Rubisco. In C<sub>2</sub> plants, this mechanism is based on tissue‐specific accumulation of glycine decarboxylase (GDC) in bundle sheath (BS) cells, relative to global expression in the cells of C<sub>3</sub> leaves. This limits photorespiratory CO<sub>2</sub> release to BS cells. Because BS cells are surrounded by photosynthetically active mesophyll cells, this arrangement enhances the probability of re‐fixation of CO<sub>2</sub>. The restriction of GDC to BS cells was mainly achieved by confinement of its P‐subunit (GLDP). Here, we provide a mechanism for the establishment of C<sub>2</sub>‐type gene expression by studying the upstream sequences of C<sub>3</sub> <em>Gldp</em> genes in <em>Arabidopsis thaliana</em>. Deletion of 59 bp in the upstream region of <em>AtGldp1</em> restricted expression of a reporter gene to BS cells and the vasculature without affecting diurnal variation. This region was named the ‘M box’. Similar results were obtained for the <em>AtGldp2</em> gene. Fusion of the M box to endogenous or exogenous promoters supported mesophyll expression. Nucleosome densities at the M box were low, suggesting an open chromatin structure facilitating transcription factor binding. <em>In silico</em> analysis defined a possible consensus for the element that was conserved across the Brassicaceae, but not in <em>Moricandia nitens</em>, a C<sub>2</sub> plant. Collective results provide evidence that a simple mutation is sufficient for establishment of C<sub>2</sub>‐specific gene expression in a C<sub>3</sub> plant.</p> |
| first_indexed | 2024-03-07T04:00:12Z |
| format | Journal article |
| id | oxford-uuid:c4500d89-26cd-4f5f-b367-3c4a9ace9c1e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:00:12Z |
| publishDate | 2015 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:c4500d89-26cd-4f5f-b367-3c4a9ace9c1e2022-03-27T06:22:37ZA simple mechanism for the establishment of C2‐specific gene expression in BrassicaceaeJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c4500d89-26cd-4f5f-b367-3c4a9ace9c1eEnglishSymplectic ElementsWiley2015Adwy, WLaxa, MPeterhansel, C<p>The transition of C<sub>3</sub>, via C<sub>2</sub> towards C<sub>4</sub> photosynthesis is an important example of stepwise evolution of a complex genetic trait. A common feature that was gradually emphasized during this trajectory is the evolution of a CO<sub>2</sub> concentration mechanism around Rubisco. In C<sub>2</sub> plants, this mechanism is based on tissue‐specific accumulation of glycine decarboxylase (GDC) in bundle sheath (BS) cells, relative to global expression in the cells of C<sub>3</sub> leaves. This limits photorespiratory CO<sub>2</sub> release to BS cells. Because BS cells are surrounded by photosynthetically active mesophyll cells, this arrangement enhances the probability of re‐fixation of CO<sub>2</sub>. The restriction of GDC to BS cells was mainly achieved by confinement of its P‐subunit (GLDP). Here, we provide a mechanism for the establishment of C<sub>2</sub>‐type gene expression by studying the upstream sequences of C<sub>3</sub> <em>Gldp</em> genes in <em>Arabidopsis thaliana</em>. Deletion of 59 bp in the upstream region of <em>AtGldp1</em> restricted expression of a reporter gene to BS cells and the vasculature without affecting diurnal variation. This region was named the ‘M box’. Similar results were obtained for the <em>AtGldp2</em> gene. Fusion of the M box to endogenous or exogenous promoters supported mesophyll expression. Nucleosome densities at the M box were low, suggesting an open chromatin structure facilitating transcription factor binding. <em>In silico</em> analysis defined a possible consensus for the element that was conserved across the Brassicaceae, but not in <em>Moricandia nitens</em>, a C<sub>2</sub> plant. Collective results provide evidence that a simple mutation is sufficient for establishment of C<sub>2</sub>‐specific gene expression in a C<sub>3</sub> plant.</p> |
| spellingShingle | Adwy, W Laxa, M Peterhansel, C A simple mechanism for the establishment of C2‐specific gene expression in Brassicaceae |
| title | A simple mechanism for the establishment of C2‐specific gene expression in Brassicaceae |
| title_full | A simple mechanism for the establishment of C2‐specific gene expression in Brassicaceae |
| title_fullStr | A simple mechanism for the establishment of C2‐specific gene expression in Brassicaceae |
| title_full_unstemmed | A simple mechanism for the establishment of C2‐specific gene expression in Brassicaceae |
| title_short | A simple mechanism for the establishment of C2‐specific gene expression in Brassicaceae |
| title_sort | simple mechanism for the establishment of c2 specific gene expression in brassicaceae |
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