Vibronic dynamics of the ultrafast all-trans to 13-cis photoisomerization of retinal in channelrhodopsin-1
Channelrhodopsins are light-gated ion channels with extensive applications in optogenetics. Channelrhodopsin-1 from Chlamydomonas augustae (CaChR1) exhibits a red-shifted absorption spectrum as compared to Channelrhodopsin-2, which is highly beneficial for optogenetic application. The primary event...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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American Chemical Society
2016
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| _version_ | 1826295420485107712 |
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| author | Schnedermann, C Muders, V Ehrenberg, D Schlesinger, R Kukura, P Heberle, J |
| author_facet | Schnedermann, C Muders, V Ehrenberg, D Schlesinger, R Kukura, P Heberle, J |
| author_sort | Schnedermann, C |
| collection | OXFORD |
| description | Channelrhodopsins are light-gated ion channels with extensive applications in optogenetics. Channelrhodopsin-1 from Chlamydomonas augustae (CaChR1) exhibits a red-shifted absorption spectrum as compared to Channelrhodopsin-2, which is highly beneficial for optogenetic application. The primary event in the photocycle of CaChR1 involves an isomerization of the protein-bound retinal chromophore. Here, we apply highly time-resolved vibronic spectroscopy to reveal the electronic and structural dynamics associated with the first step of the photocycle of CaChR1. We observe vibrationally coherent formation of the P1 intermediate exhibiting a twisted 13-cis retinal with a 110 ± 7 fs time constant. Comparison with low-temperature resonance Raman spectroscopy of the corresponding trapped photoproduct demonstrates that this rapidly formed P1 intermediate is stable for several hundreds of nanoseconds. |
| first_indexed | 2024-03-07T04:00:46Z |
| format | Journal article |
| id | oxford-uuid:c48089f7-7db1-442c-a47c-4e20b1d55ce3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:00:46Z |
| publishDate | 2016 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:c48089f7-7db1-442c-a47c-4e20b1d55ce32022-03-27T06:23:51ZVibronic dynamics of the ultrafast all-trans to 13-cis photoisomerization of retinal in channelrhodopsin-1Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c48089f7-7db1-442c-a47c-4e20b1d55ce3EnglishSymplectic Elements at OxfordAmerican Chemical Society2016Schnedermann, CMuders, VEhrenberg, DSchlesinger, RKukura, PHeberle, JChannelrhodopsins are light-gated ion channels with extensive applications in optogenetics. Channelrhodopsin-1 from Chlamydomonas augustae (CaChR1) exhibits a red-shifted absorption spectrum as compared to Channelrhodopsin-2, which is highly beneficial for optogenetic application. The primary event in the photocycle of CaChR1 involves an isomerization of the protein-bound retinal chromophore. Here, we apply highly time-resolved vibronic spectroscopy to reveal the electronic and structural dynamics associated with the first step of the photocycle of CaChR1. We observe vibrationally coherent formation of the P1 intermediate exhibiting a twisted 13-cis retinal with a 110 ± 7 fs time constant. Comparison with low-temperature resonance Raman spectroscopy of the corresponding trapped photoproduct demonstrates that this rapidly formed P1 intermediate is stable for several hundreds of nanoseconds. |
| spellingShingle | Schnedermann, C Muders, V Ehrenberg, D Schlesinger, R Kukura, P Heberle, J Vibronic dynamics of the ultrafast all-trans to 13-cis photoisomerization of retinal in channelrhodopsin-1 |
| title | Vibronic dynamics of the ultrafast all-trans to 13-cis photoisomerization of retinal in channelrhodopsin-1 |
| title_full | Vibronic dynamics of the ultrafast all-trans to 13-cis photoisomerization of retinal in channelrhodopsin-1 |
| title_fullStr | Vibronic dynamics of the ultrafast all-trans to 13-cis photoisomerization of retinal in channelrhodopsin-1 |
| title_full_unstemmed | Vibronic dynamics of the ultrafast all-trans to 13-cis photoisomerization of retinal in channelrhodopsin-1 |
| title_short | Vibronic dynamics of the ultrafast all-trans to 13-cis photoisomerization of retinal in channelrhodopsin-1 |
| title_sort | vibronic dynamics of the ultrafast all trans to 13 cis photoisomerization of retinal in channelrhodopsin 1 |
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