Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates from Yaoundé, Cameroon, 2019 to 2020
<p>This study investigated antimicrobial resistance (AMR) phenotypes and genotypes exhibited by Neisseria gonorrhoeae from Yaoundé, Cameroon. AMR to tetracycline, penicillin and ciprofloxacin was observed although none of the isolates had reduced susceptibility to...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Microbiology Society
2023
|
| _version_ | 1797111742153621504 |
|---|---|
| author | Tayimetha, CY Njunda, LA Akenji, B Founou, RC Feteh, V Zofou, D Chafa, A Oyono, Y Etogo, B Tseuko, D Fonkoua, MC Harrison, OB |
| author_facet | Tayimetha, CY Njunda, LA Akenji, B Founou, RC Feteh, V Zofou, D Chafa, A Oyono, Y Etogo, B Tseuko, D Fonkoua, MC Harrison, OB |
| author_sort | Tayimetha, CY |
| collection | OXFORD |
| description | <p>This study investigated antimicrobial resistance (AMR) phenotypes and genotypes exhibited by Neisseria gonorrhoeae from Yaoundé, Cameroon. AMR to tetracycline, penicillin and ciprofloxacin was observed although none of the isolates had reduced susceptibility to azithromycin, cefixime or ceftriaxone. Whole genome sequence (WGS) data were obtained and, using a threshold of 300 or fewer locus differences in the N. gonorrhoeae core gene multilocus sequence typing (cgMLST) scheme, four distinct core genome lineages were identified. Publicly available WGS data from 1355 gonococci belonging to these four lineages were retrieved from the PubMLST database, allowing the Cameroonian isolates to be examined in the context of existing data and compared with related gonococci. Examination of AMR genotypes in this dataset found an association between the core genome and AMR with, for example, isolates belonging to the core genome group, Ng_cgc_300 : 21, possessing GyrA and ParC alleles with amino acid substitutions conferring high-level resistance to ciprofloxacin while lineages Ng_cgc_300 : 41 and Ng_cgc_300 : 243 were predicted to be susceptible to several antimicrobials. A core genome lineage, Ng_cgc_300 : 498, was observed which largely consisted of gonococci originating from Africa. Analyses from this study demonstrate the advantages of using the N. gonorrhoeae cgMLST scheme to find related gonococci to carry out genomic analyses that enhance our understanding of the population biology of this important pathogen.</p> |
| first_indexed | 2024-03-07T08:14:37Z |
| format | Journal article |
| id | oxford-uuid:c4a6e524-eb1c-41ff-b011-826f78fcd330 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:14:37Z |
| publishDate | 2023 |
| publisher | Microbiology Society |
| record_format | dspace |
| spelling | oxford-uuid:c4a6e524-eb1c-41ff-b011-826f78fcd3302023-12-13T14:42:07ZPhenotypic and genotypic characterization of Neisseria gonorrhoeae isolates from Yaoundé, Cameroon, 2019 to 2020Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c4a6e524-eb1c-41ff-b011-826f78fcd330EnglishSymplectic ElementsMicrobiology Society2023Tayimetha, CYNjunda, LAAkenji, BFounou, RCFeteh, VZofou, DChafa, AOyono, YEtogo, BTseuko, DFonkoua, MCHarrison, OB<p>This study investigated antimicrobial resistance (AMR) phenotypes and genotypes exhibited by Neisseria gonorrhoeae from Yaoundé, Cameroon. AMR to tetracycline, penicillin and ciprofloxacin was observed although none of the isolates had reduced susceptibility to azithromycin, cefixime or ceftriaxone. Whole genome sequence (WGS) data were obtained and, using a threshold of 300 or fewer locus differences in the N. gonorrhoeae core gene multilocus sequence typing (cgMLST) scheme, four distinct core genome lineages were identified. Publicly available WGS data from 1355 gonococci belonging to these four lineages were retrieved from the PubMLST database, allowing the Cameroonian isolates to be examined in the context of existing data and compared with related gonococci. Examination of AMR genotypes in this dataset found an association between the core genome and AMR with, for example, isolates belonging to the core genome group, Ng_cgc_300 : 21, possessing GyrA and ParC alleles with amino acid substitutions conferring high-level resistance to ciprofloxacin while lineages Ng_cgc_300 : 41 and Ng_cgc_300 : 243 were predicted to be susceptible to several antimicrobials. A core genome lineage, Ng_cgc_300 : 498, was observed which largely consisted of gonococci originating from Africa. Analyses from this study demonstrate the advantages of using the N. gonorrhoeae cgMLST scheme to find related gonococci to carry out genomic analyses that enhance our understanding of the population biology of this important pathogen.</p> |
| spellingShingle | Tayimetha, CY Njunda, LA Akenji, B Founou, RC Feteh, V Zofou, D Chafa, A Oyono, Y Etogo, B Tseuko, D Fonkoua, MC Harrison, OB Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates from Yaoundé, Cameroon, 2019 to 2020 |
| title | Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates from Yaoundé, Cameroon, 2019 to 2020 |
| title_full | Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates from Yaoundé, Cameroon, 2019 to 2020 |
| title_fullStr | Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates from Yaoundé, Cameroon, 2019 to 2020 |
| title_full_unstemmed | Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates from Yaoundé, Cameroon, 2019 to 2020 |
| title_short | Phenotypic and genotypic characterization of Neisseria gonorrhoeae isolates from Yaoundé, Cameroon, 2019 to 2020 |
| title_sort | phenotypic and genotypic characterization of neisseria gonorrhoeae isolates from yaounde cameroon 2019 to 2020 |
| work_keys_str_mv | AT tayimethacy phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT njundala phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT akenjib phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT founourc phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT fetehv phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT zofoud phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT chafaa phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT oyonoy phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT etogob phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT tseukod phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT fonkouamc phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 AT harrisonob phenotypicandgenotypiccharacterizationofneisseriagonorrhoeaeisolatesfromyaoundecameroon2019to2020 |