Isolation and expansion of cardiosphere-derived stem cells
The isolation and in vitro expansion of stem cells from the adult heart may provide a cell therapy for regenerating damaged myocardium. Cardiac stem cells can be isolated via magnetic or fluorescent cell sorting using specific cell-surface markers, including c-kit, Sca-1, and Isl-1. Because these is...
| Үндсэн зохиолчид: | , , , , , , |
|---|---|
| Формат: | Journal article |
| Хэвлэсэн: |
2011
|
| _version_ | 1826295459426074624 |
|---|---|
| author | Tan, J Carr, C Stuckey, D Clarke, K Ellison, G Messina, E Giacomello, A |
| author_facet | Tan, J Carr, C Stuckey, D Clarke, K Ellison, G Messina, E Giacomello, A |
| author_sort | Tan, J |
| collection | OXFORD |
| description | The isolation and in vitro expansion of stem cells from the adult heart may provide a cell therapy for regenerating damaged myocardium. Cardiac stem cells can be isolated via magnetic or fluorescent cell sorting using specific cell-surface markers, including c-kit, Sca-1, and Isl-1. Because these isolation methods yield relatively few cells, substantial in vitro expansion is required to generate sufficient cell numbers for therapy. An alternative method uses cells spontaneously shed from cultured heart explants, which are harvested, induced to form cardiospheres, and expanded as a monolayer for several passages. This method for generating therapeutically relevant numbers of cells in a shorter time period than cell surface marker-based isolations is ideally suited for autologous cardiac stem cell therapy after myocardial infarction. © 2011 by John Wiley and Sons, Inc. |
| first_indexed | 2024-03-07T04:01:22Z |
| format | Journal article |
| id | oxford-uuid:c4aef359-d8f4-4a6c-9bee-39b2ea898fd1 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T04:01:22Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:c4aef359-d8f4-4a6c-9bee-39b2ea898fd12022-03-27T06:25:22ZIsolation and expansion of cardiosphere-derived stem cellsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c4aef359-d8f4-4a6c-9bee-39b2ea898fd1Symplectic Elements at Oxford2011Tan, JCarr, CStuckey, DClarke, KEllison, GMessina, EGiacomello, AThe isolation and in vitro expansion of stem cells from the adult heart may provide a cell therapy for regenerating damaged myocardium. Cardiac stem cells can be isolated via magnetic or fluorescent cell sorting using specific cell-surface markers, including c-kit, Sca-1, and Isl-1. Because these isolation methods yield relatively few cells, substantial in vitro expansion is required to generate sufficient cell numbers for therapy. An alternative method uses cells spontaneously shed from cultured heart explants, which are harvested, induced to form cardiospheres, and expanded as a monolayer for several passages. This method for generating therapeutically relevant numbers of cells in a shorter time period than cell surface marker-based isolations is ideally suited for autologous cardiac stem cell therapy after myocardial infarction. © 2011 by John Wiley and Sons, Inc. |
| spellingShingle | Tan, J Carr, C Stuckey, D Clarke, K Ellison, G Messina, E Giacomello, A Isolation and expansion of cardiosphere-derived stem cells |
| title | Isolation and expansion of cardiosphere-derived stem cells |
| title_full | Isolation and expansion of cardiosphere-derived stem cells |
| title_fullStr | Isolation and expansion of cardiosphere-derived stem cells |
| title_full_unstemmed | Isolation and expansion of cardiosphere-derived stem cells |
| title_short | Isolation and expansion of cardiosphere-derived stem cells |
| title_sort | isolation and expansion of cardiosphere derived stem cells |
| work_keys_str_mv | AT tanj isolationandexpansionofcardiospherederivedstemcells AT carrc isolationandexpansionofcardiospherederivedstemcells AT stuckeyd isolationandexpansionofcardiospherederivedstemcells AT clarkek isolationandexpansionofcardiospherederivedstemcells AT ellisong isolationandexpansionofcardiospherederivedstemcells AT messinae isolationandexpansionofcardiospherederivedstemcells AT giacomelloa isolationandexpansionofcardiospherederivedstemcells |