An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
The exit of antigen-presenting cells and lymphocytes from inflamed skin to afferent lymph is vital for the initiation and maintenance of dermal immune responses. How such an exit is achieved and how cells transmigrate the distinct endothelium of lymphatic vessels are unknown. We show that inflammato...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
2006
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| _version_ | 1826295616483885056 |
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| author | Johnson, L Clasper, S Holt, A Lalor, P Baban, D Jackson, D |
| author_facet | Johnson, L Clasper, S Holt, A Lalor, P Baban, D Jackson, D |
| author_sort | Johnson, L |
| collection | OXFORD |
| description | The exit of antigen-presenting cells and lymphocytes from inflamed skin to afferent lymph is vital for the initiation and maintenance of dermal immune responses. How such an exit is achieved and how cells transmigrate the distinct endothelium of lymphatic vessels are unknown. We show that inflammatory cytokines trigger activation of dermal lymphatic endothelial cells (LECs), leading to expression of the key leukocyte adhesion receptors intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin, as well as a discrete panel of chemokines and other potential regulators of leukocyte transmigration. Furthermore, we show that both ICAM-1 and VCAM-1 are induced in the dermal lymphatic vessels of mice exposed to skin contact hypersensitivity where they mediate lymph node trafficking of dendritic cells (DCs) via afferent lymphatics. Lastly, we show that tumor necrosis factor alpha stimulates both DC adhesion and transmigration of dermal LEC monolayers in vitro and that the process is efficiently inhibited by ICAM-1 and VCAM-1 adhesion-blocking monoclonal antibodies. These results reveal a CAM-mediated mechanism for recruiting leukocytes to the lymph nodes in inflammation and highlight the process of lymphatic transmigration as a potential new target for antiinflammatory therapy. |
| first_indexed | 2024-03-07T04:03:47Z |
| format | Journal article |
| id | oxford-uuid:c576ad20-e4bb-42fb-a6bc-b3858d5bcc40 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:03:47Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:c576ad20-e4bb-42fb-a6bc-b3858d5bcc402022-03-27T06:31:06ZAn inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c576ad20-e4bb-42fb-a6bc-b3858d5bcc40EnglishSymplectic Elements at Oxford2006Johnson, LClasper, SHolt, ALalor, PBaban, DJackson, DThe exit of antigen-presenting cells and lymphocytes from inflamed skin to afferent lymph is vital for the initiation and maintenance of dermal immune responses. How such an exit is achieved and how cells transmigrate the distinct endothelium of lymphatic vessels are unknown. We show that inflammatory cytokines trigger activation of dermal lymphatic endothelial cells (LECs), leading to expression of the key leukocyte adhesion receptors intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and E-selectin, as well as a discrete panel of chemokines and other potential regulators of leukocyte transmigration. Furthermore, we show that both ICAM-1 and VCAM-1 are induced in the dermal lymphatic vessels of mice exposed to skin contact hypersensitivity where they mediate lymph node trafficking of dendritic cells (DCs) via afferent lymphatics. Lastly, we show that tumor necrosis factor alpha stimulates both DC adhesion and transmigration of dermal LEC monolayers in vitro and that the process is efficiently inhibited by ICAM-1 and VCAM-1 adhesion-blocking monoclonal antibodies. These results reveal a CAM-mediated mechanism for recruiting leukocytes to the lymph nodes in inflammation and highlight the process of lymphatic transmigration as a potential new target for antiinflammatory therapy. |
| spellingShingle | Johnson, L Clasper, S Holt, A Lalor, P Baban, D Jackson, D An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. |
| title | An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. |
| title_full | An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. |
| title_fullStr | An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. |
| title_full_unstemmed | An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. |
| title_short | An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. |
| title_sort | inflammation induced mechanism for leukocyte transmigration across lymphatic vessel endothelium |
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