Selective dendritic localization of mRNA in Drosophila mushroom body output neurons.
Memory-relevant neuronal plasticity is believed to require local translation of new proteins at synapses. Understanding this process requires the visualization of the relevant mRNAs within these neuronal compartments. Here, we used single-molecule fluorescence in situ hybridization to localize mRNAs...
| Asıl Yazarlar: | , , , , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
eLife Sciences Publications
2021
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| _version_ | 1826295644341403648 |
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| author | Mitchell, J Smith, CS Titlow, J Otto, N van Velde, P Booth, M Davis, I Waddell, S |
| author_facet | Mitchell, J Smith, CS Titlow, J Otto, N van Velde, P Booth, M Davis, I Waddell, S |
| author_sort | Mitchell, J |
| collection | OXFORD |
| description | Memory-relevant neuronal plasticity is believed to require local translation of new proteins at synapses. Understanding this process requires the visualization of the relevant mRNAs within these neuronal compartments. Here, we used single-molecule fluorescence in situ hybridization to localize mRNAs at subcellular resolution in the adult <i>Drosophila</i> brain. mRNAs for subunits of nicotinic acetylcholine receptors and kinases could be detected within the dendrites of co-labeled mushroom body output neurons (MBONs) and their relative abundance showed cell specificity. Moreover, aversive olfactory learning produced a transient increase in the level of CaMKII mRNA within the dendritic compartments of the γ5β'2a MBONs. Localization of specific mRNAs in MBONs before and after learning represents a critical step towards deciphering the role of dendritic translation in the neuronal plasticity underlying behavioral change in <i>Drosophila</i>. |
| first_indexed | 2024-03-07T04:04:13Z |
| format | Journal article |
| id | oxford-uuid:c5967025-26dd-4b6b-a4e7-e288e32593c0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:04:13Z |
| publishDate | 2021 |
| publisher | eLife Sciences Publications |
| record_format | dspace |
| spelling | oxford-uuid:c5967025-26dd-4b6b-a4e7-e288e32593c02022-03-27T06:32:13ZSelective dendritic localization of mRNA in Drosophila mushroom body output neurons.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c5967025-26dd-4b6b-a4e7-e288e32593c0EnglishSymplectic ElementseLife Sciences Publications2021Mitchell, JSmith, CSTitlow, JOtto, Nvan Velde, PBooth, MDavis, IWaddell, SMemory-relevant neuronal plasticity is believed to require local translation of new proteins at synapses. Understanding this process requires the visualization of the relevant mRNAs within these neuronal compartments. Here, we used single-molecule fluorescence in situ hybridization to localize mRNAs at subcellular resolution in the adult <i>Drosophila</i> brain. mRNAs for subunits of nicotinic acetylcholine receptors and kinases could be detected within the dendrites of co-labeled mushroom body output neurons (MBONs) and their relative abundance showed cell specificity. Moreover, aversive olfactory learning produced a transient increase in the level of CaMKII mRNA within the dendritic compartments of the γ5β'2a MBONs. Localization of specific mRNAs in MBONs before and after learning represents a critical step towards deciphering the role of dendritic translation in the neuronal plasticity underlying behavioral change in <i>Drosophila</i>. |
| spellingShingle | Mitchell, J Smith, CS Titlow, J Otto, N van Velde, P Booth, M Davis, I Waddell, S Selective dendritic localization of mRNA in Drosophila mushroom body output neurons. |
| title | Selective dendritic localization of mRNA in Drosophila mushroom body output neurons. |
| title_full | Selective dendritic localization of mRNA in Drosophila mushroom body output neurons. |
| title_fullStr | Selective dendritic localization of mRNA in Drosophila mushroom body output neurons. |
| title_full_unstemmed | Selective dendritic localization of mRNA in Drosophila mushroom body output neurons. |
| title_short | Selective dendritic localization of mRNA in Drosophila mushroom body output neurons. |
| title_sort | selective dendritic localization of mrna in drosophila mushroom body output neurons |
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