Long-read single-cell sequencing using scCOLOR-seq
Single-cell sequencing allows for the measurement of sequence information from individual cells with next-generation sequencing (NGS). However, its application to third-generation sequencing platforms such as Oxford Nanopore has been challenging because of its lower basecalling accuracy. Here we des...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Book section |
| Language: | English |
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Humana Press
2023
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| _version_ | 1797111630156267520 |
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| author | Philpott, M Oppermann, U Cribbs, AP |
| author2 | Arakawa, K |
| author_facet | Arakawa, K Philpott, M Oppermann, U Cribbs, AP |
| author_sort | Philpott, M |
| collection | OXFORD |
| description | Single-cell sequencing allows for the measurement of sequence information from individual cells with next-generation sequencing (NGS). However, its application to third-generation sequencing platforms such as Oxford Nanopore has been challenging because of its lower basecalling accuracy. Here we describe the method to perform highly accurate single-cell COrrected Long-Read sequencing (scCOLOR-seq) by droplet-based encapsulation of cells and sequencing using the Oxford Nanopore Sequencing system. |
| first_indexed | 2024-03-07T08:13:08Z |
| format | Book section |
| id | oxford-uuid:c5c6ef8c-03ea-4b74-9fec-7e13c1f92658 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:13:08Z |
| publishDate | 2023 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:c5c6ef8c-03ea-4b74-9fec-7e13c1f926582023-12-05T14:04:03ZLong-read single-cell sequencing using scCOLOR-seqBook sectionhttp://purl.org/coar/resource_type/c_1843uuid:c5c6ef8c-03ea-4b74-9fec-7e13c1f92658EnglishSymplectic ElementsHumana Press2023Philpott, MOppermann, UCribbs, APArakawa, KSingle-cell sequencing allows for the measurement of sequence information from individual cells with next-generation sequencing (NGS). However, its application to third-generation sequencing platforms such as Oxford Nanopore has been challenging because of its lower basecalling accuracy. Here we describe the method to perform highly accurate single-cell COrrected Long-Read sequencing (scCOLOR-seq) by droplet-based encapsulation of cells and sequencing using the Oxford Nanopore Sequencing system. |
| spellingShingle | Philpott, M Oppermann, U Cribbs, AP Long-read single-cell sequencing using scCOLOR-seq |
| title | Long-read single-cell sequencing using scCOLOR-seq |
| title_full | Long-read single-cell sequencing using scCOLOR-seq |
| title_fullStr | Long-read single-cell sequencing using scCOLOR-seq |
| title_full_unstemmed | Long-read single-cell sequencing using scCOLOR-seq |
| title_short | Long-read single-cell sequencing using scCOLOR-seq |
| title_sort | long read single cell sequencing using sccolor seq |
| work_keys_str_mv | AT philpottm longreadsinglecellsequencingusingsccolorseq AT oppermannu longreadsinglecellsequencingusingsccolorseq AT cribbsap longreadsinglecellsequencingusingsccolorseq |