Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues.
The tricarboxylic acid (TCA) cycle is a crucial component of respiratory metabolism in both photosynthetic and heterotrophic plant organs. All of the major genes of the tomato TCA cycle have been cloned recently, allowing the generation of a suite of transgenic plants in which the majority of the en...
| मुख्य लेखकों: | , , , , |
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| स्वरूप: | Journal article |
| भाषा: | English |
| प्रकाशित: |
2012
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| _version_ | 1826295731495895040 |
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| author | Araújo, W Nunes-Nesi, A Nikoloski, Z Sweetlove, L Fernie, A |
| author_facet | Araújo, W Nunes-Nesi, A Nikoloski, Z Sweetlove, L Fernie, A |
| author_sort | Araújo, W |
| collection | OXFORD |
| description | The tricarboxylic acid (TCA) cycle is a crucial component of respiratory metabolism in both photosynthetic and heterotrophic plant organs. All of the major genes of the tomato TCA cycle have been cloned recently, allowing the generation of a suite of transgenic plants in which the majority of the enzymes in the pathway are progressively decreased. Investigations of these plants have provided an almost complete view of the distribution of control in this important pathway. Our studies suggest that citrate synthase, aconitase, isocitrate dehydrogenase, succinyl CoA ligase, succinate dehydrogenase, fumarase and malate dehydrogenase have control coefficients flux for respiration of -0.4, 0.964, -0.123, 0.0008, 0.289, 0.601 and 1.76, respectively; while 2-oxoglutarate dehydrogenase is estimated to have a control coefficient of 0.786 in potato tubers. These results thus indicate that the control of this pathway is distributed among malate dehydrogenase, aconitase, fumarase, succinate dehydrogenase and 2-oxoglutarate dehydrogenase. The unusual distribution of control estimated here is consistent with specific non-cyclic flux mode and cytosolic bypasses that operate in illuminated leaves. These observations are discussed in the context of known regulatory properties of the enzymes and some illustrative examples of how the pathway responds to environmental change are given. |
| first_indexed | 2024-03-07T04:05:34Z |
| format | Journal article |
| id | oxford-uuid:c6052201-c294-4411-a5fc-f9ac44ecbf8a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:05:34Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:c6052201-c294-4411-a5fc-f9ac44ecbf8a2022-03-27T06:35:13ZMetabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c6052201-c294-4411-a5fc-f9ac44ecbf8aEnglishSymplectic Elements at Oxford2012Araújo, WNunes-Nesi, ANikoloski, ZSweetlove, LFernie, AThe tricarboxylic acid (TCA) cycle is a crucial component of respiratory metabolism in both photosynthetic and heterotrophic plant organs. All of the major genes of the tomato TCA cycle have been cloned recently, allowing the generation of a suite of transgenic plants in which the majority of the enzymes in the pathway are progressively decreased. Investigations of these plants have provided an almost complete view of the distribution of control in this important pathway. Our studies suggest that citrate synthase, aconitase, isocitrate dehydrogenase, succinyl CoA ligase, succinate dehydrogenase, fumarase and malate dehydrogenase have control coefficients flux for respiration of -0.4, 0.964, -0.123, 0.0008, 0.289, 0.601 and 1.76, respectively; while 2-oxoglutarate dehydrogenase is estimated to have a control coefficient of 0.786 in potato tubers. These results thus indicate that the control of this pathway is distributed among malate dehydrogenase, aconitase, fumarase, succinate dehydrogenase and 2-oxoglutarate dehydrogenase. The unusual distribution of control estimated here is consistent with specific non-cyclic flux mode and cytosolic bypasses that operate in illuminated leaves. These observations are discussed in the context of known regulatory properties of the enzymes and some illustrative examples of how the pathway responds to environmental change are given. |
| spellingShingle | Araújo, W Nunes-Nesi, A Nikoloski, Z Sweetlove, L Fernie, A Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues. |
| title | Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues. |
| title_full | Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues. |
| title_fullStr | Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues. |
| title_full_unstemmed | Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues. |
| title_short | Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues. |
| title_sort | metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues |
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