Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies.
In an attempt to improve the diagnostic value of measuring antibodies to islet cell cytoplasmic antigen, coded sera were distributed to 38 laboratories and results were returned for analysis. Comparison between laboratories revealed that results for some individual sera differed by up to nine doubli...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1988
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| _version_ | 1826295762674253824 |
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| author | Bonifacio, E Lernmark, A Dawkins, R |
| author_facet | Bonifacio, E Lernmark, A Dawkins, R |
| author_sort | Bonifacio, E |
| collection | OXFORD |
| description | In an attempt to improve the diagnostic value of measuring antibodies to islet cell cytoplasmic antigen, coded sera were distributed to 38 laboratories and results were returned for analysis. Comparison between laboratories revealed that results for some individual sera differed by up to nine doubling dilutions and even within laboratories duplicate samples could differ by six doubling dilutions. By including dilutions of sera it was possible to draw a standard curve for each laboratory and this revealed major variations in shape, slope and intercept. However, using each laboratory's standard curve and converting results to units, a substantial improvement was obtained. The approach described improves standardisation and will permit laboratories to identify poor precision and/or any systematic change in assay performance. |
| first_indexed | 2024-03-07T04:06:02Z |
| format | Journal article |
| id | oxford-uuid:c6301922-71fe-4255-a9df-441cd1a4add7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:06:02Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:c6301922-71fe-4255-a9df-441cd1a4add72022-03-27T06:36:23ZSerum exchange and use of dilutions have improved precision of measurement of islet cell antibodies.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c6301922-71fe-4255-a9df-441cd1a4add7EnglishSymplectic Elements at Oxford1988Bonifacio, ELernmark, ADawkins, RIn an attempt to improve the diagnostic value of measuring antibodies to islet cell cytoplasmic antigen, coded sera were distributed to 38 laboratories and results were returned for analysis. Comparison between laboratories revealed that results for some individual sera differed by up to nine doubling dilutions and even within laboratories duplicate samples could differ by six doubling dilutions. By including dilutions of sera it was possible to draw a standard curve for each laboratory and this revealed major variations in shape, slope and intercept. However, using each laboratory's standard curve and converting results to units, a substantial improvement was obtained. The approach described improves standardisation and will permit laboratories to identify poor precision and/or any systematic change in assay performance. |
| spellingShingle | Bonifacio, E Lernmark, A Dawkins, R Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies. |
| title | Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies. |
| title_full | Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies. |
| title_fullStr | Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies. |
| title_full_unstemmed | Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies. |
| title_short | Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies. |
| title_sort | serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies |
| work_keys_str_mv | AT bonifacioe serumexchangeanduseofdilutionshaveimprovedprecisionofmeasurementofisletcellantibodies AT lernmarka serumexchangeanduseofdilutionshaveimprovedprecisionofmeasurementofisletcellantibodies AT dawkinsr serumexchangeanduseofdilutionshaveimprovedprecisionofmeasurementofisletcellantibodies |