Small vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencing
<p>Gene expression can be regulated by transcriptional or post-transcriptional gene silencing. Recently, we described nuclear nascent RNA silencing that is mediated by Dicer-dependent tRNA-derived small RNA molecules. In addition to tRNA, RNA polymerase III also transcribes vault RNA, a compon...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Rockefeller University Press
2023
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| _version_ | 1797110217820864512 |
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| author | Alagia, A Tereňová, J Ketley, RF Di Fazio, A Chelysheva, I Gullerova, M |
| author_facet | Alagia, A Tereňová, J Ketley, RF Di Fazio, A Chelysheva, I Gullerova, M |
| author_sort | Alagia, A |
| collection | OXFORD |
| description | <p>Gene expression can be regulated by transcriptional or post-transcriptional gene silencing. Recently, we described nuclear nascent RNA silencing that is mediated by Dicer-dependent tRNA-derived small RNA molecules. In addition to tRNA, RNA polymerase III also transcribes vault RNA, a component of the ribonucleoprotein complex vault. Here, we show that Dicer-dependent small vault RNA1-2 (svtRNA1-2) associates with Argonaute 2 (Ago2). Although endogenous vtRNA1-2 is present mostly in the cytoplasm, svtRNA1-2 localises predominantly in the nucleus. Furthermore, in Ago2 and Dicer knockdown cells, a subset of genes that are up-regulated at the nascent level were predicted to be targeted by svtRNA1-2 in the intronic region. Genomic deletion of vtRNA1-2 results in impaired cellular proliferation and the up-regulation of genes associated with cell membrane physiology and cell adhesion. Silencing activity of svtRNA1-2 molecules is dependent on seed-plus-complementary-paired hybridisation features and the presence of a 5-nucleotide loop protrusion on target RNAs. Our data reveal a role of Dicer-dependent svtRNA1-2, possessing unique molecular features, in modulation of the expression of membrane-associated proteins at the nascent RNA level.</p> |
| first_indexed | 2024-03-07T07:52:07Z |
| format | Journal article |
| id | oxford-uuid:c655fdc9-3574-4635-9733-519b78bc04a1 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:52:07Z |
| publishDate | 2023 |
| publisher | Rockefeller University Press |
| record_format | dspace |
| spelling | oxford-uuid:c655fdc9-3574-4635-9733-519b78bc04a12023-07-21T11:48:34ZSmall vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencingJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c655fdc9-3574-4635-9733-519b78bc04a1EnglishSymplectic ElementsRockefeller University Press 2023Alagia, ATereňová, JKetley, RFDi Fazio, AChelysheva, IGullerova, M<p>Gene expression can be regulated by transcriptional or post-transcriptional gene silencing. Recently, we described nuclear nascent RNA silencing that is mediated by Dicer-dependent tRNA-derived small RNA molecules. In addition to tRNA, RNA polymerase III also transcribes vault RNA, a component of the ribonucleoprotein complex vault. Here, we show that Dicer-dependent small vault RNA1-2 (svtRNA1-2) associates with Argonaute 2 (Ago2). Although endogenous vtRNA1-2 is present mostly in the cytoplasm, svtRNA1-2 localises predominantly in the nucleus. Furthermore, in Ago2 and Dicer knockdown cells, a subset of genes that are up-regulated at the nascent level were predicted to be targeted by svtRNA1-2 in the intronic region. Genomic deletion of vtRNA1-2 results in impaired cellular proliferation and the up-regulation of genes associated with cell membrane physiology and cell adhesion. Silencing activity of svtRNA1-2 molecules is dependent on seed-plus-complementary-paired hybridisation features and the presence of a 5-nucleotide loop protrusion on target RNAs. Our data reveal a role of Dicer-dependent svtRNA1-2, possessing unique molecular features, in modulation of the expression of membrane-associated proteins at the nascent RNA level.</p> |
| spellingShingle | Alagia, A Tereňová, J Ketley, RF Di Fazio, A Chelysheva, I Gullerova, M Small vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencing |
| title | Small vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencing |
| title_full | Small vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencing |
| title_fullStr | Small vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencing |
| title_full_unstemmed | Small vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencing |
| title_short | Small vault RNA1-2 modulates expression of cell membrane proteins through nascent RNA silencing |
| title_sort | small vault rna1 2 modulates expression of cell membrane proteins through nascent rna silencing |
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