CD8+ T cell responses to human immunodeficiency viruses type 2 (HIV-2) and type 1 (HIV-1) gag proteins are distinguishable by magnitude and breadth but not cellular phenotype
The mechanisms underlying the relatively slow progression of human immunodeficiency virus type 2 (HIV-2) compared with HIV-1 infection are undefined and could be a result of more effective immune responses. We used HIV-2 and HIV-1 IFN-γ enzyme-linked immunospot assays to evaluate CD8+ T cell respons...
| Hlavní autoři: | , , , , , , , , , , , |
|---|---|
| Médium: | Journal article |
| Jazyk: | English |
| Vydáno: |
2005
|
| _version_ | 1826295836495052800 |
|---|---|
| author | Gillespie, G Pinheiro, S Sayeid-Al-Jamee, M Alabi, A Kaye, S Sarge-Njie, R Njai, H Joof, K Jaye, A Whittle, H Rowland-Jones, S Dorrell, L |
| author_facet | Gillespie, G Pinheiro, S Sayeid-Al-Jamee, M Alabi, A Kaye, S Sarge-Njie, R Njai, H Joof, K Jaye, A Whittle, H Rowland-Jones, S Dorrell, L |
| author_sort | Gillespie, G |
| collection | OXFORD |
| description | The mechanisms underlying the relatively slow progression of human immunodeficiency virus type 2 (HIV-2) compared with HIV-1 infection are undefined and could be a result of more effective immune responses. We used HIV-2 and HIV-1 IFN-γ enzyme-linked immunospot assays to evaluate CD8+ T cell responses in antiretroviral-naive HIV-2-('HIV-2+') and HIV-1-infected ('HIV-1+') individuals. Gag-specific responses were detected in the majority of HIV-2+ and HIV-1+ subjects. Overlapping gag peptide analysis indicated a significantly greater magnitude and breadth of responses in the HIV-1+ cohort, and this difference was attributable to low responses in HIV-2+ subjects with undetectable viral load (medians 2107 and 512 spot-forming units per 106 PBMC, respectively, p=0.007). We investigated the phenotype of viral epitope-specific CD8+ T cells identified with HLA-B53- and HLA-B58-peptide tetramers (8 HIV-2+, 11 HIV-1+ subjects). HIV-2-specific CD8+ T cells were predominantly CD27+ CD45RA-, and only a minority expressed perforin. The limited breadth and low frequency of CD8+ T cell responses to HIV-2 gag in aviremic HIV-2+ subjects suggests that these responses reflect antigen load in plasma, as is the case in HIV-1 infection. Immune control of HIV-2 does not appear to be related to the frequency of perforin-expressing virus-specific CD8+ T cells. © 2005 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. |
| first_indexed | 2024-03-07T04:07:07Z |
| format | Journal article |
| id | oxford-uuid:c68c617f-9e89-4f22-8491-8ec2357068f6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:07:07Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:c68c617f-9e89-4f22-8491-8ec2357068f62022-03-27T06:38:54ZCD8+ T cell responses to human immunodeficiency viruses type 2 (HIV-2) and type 1 (HIV-1) gag proteins are distinguishable by magnitude and breadth but not cellular phenotypeJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c68c617f-9e89-4f22-8491-8ec2357068f6EnglishSymplectic Elements at Oxford2005Gillespie, GPinheiro, SSayeid-Al-Jamee, MAlabi, AKaye, SSarge-Njie, RNjai, HJoof, KJaye, AWhittle, HRowland-Jones, SDorrell, LThe mechanisms underlying the relatively slow progression of human immunodeficiency virus type 2 (HIV-2) compared with HIV-1 infection are undefined and could be a result of more effective immune responses. We used HIV-2 and HIV-1 IFN-γ enzyme-linked immunospot assays to evaluate CD8+ T cell responses in antiretroviral-naive HIV-2-('HIV-2+') and HIV-1-infected ('HIV-1+') individuals. Gag-specific responses were detected in the majority of HIV-2+ and HIV-1+ subjects. Overlapping gag peptide analysis indicated a significantly greater magnitude and breadth of responses in the HIV-1+ cohort, and this difference was attributable to low responses in HIV-2+ subjects with undetectable viral load (medians 2107 and 512 spot-forming units per 106 PBMC, respectively, p=0.007). We investigated the phenotype of viral epitope-specific CD8+ T cells identified with HLA-B53- and HLA-B58-peptide tetramers (8 HIV-2+, 11 HIV-1+ subjects). HIV-2-specific CD8+ T cells were predominantly CD27+ CD45RA-, and only a minority expressed perforin. The limited breadth and low frequency of CD8+ T cell responses to HIV-2 gag in aviremic HIV-2+ subjects suggests that these responses reflect antigen load in plasma, as is the case in HIV-1 infection. Immune control of HIV-2 does not appear to be related to the frequency of perforin-expressing virus-specific CD8+ T cells. © 2005 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim. |
| spellingShingle | Gillespie, G Pinheiro, S Sayeid-Al-Jamee, M Alabi, A Kaye, S Sarge-Njie, R Njai, H Joof, K Jaye, A Whittle, H Rowland-Jones, S Dorrell, L CD8+ T cell responses to human immunodeficiency viruses type 2 (HIV-2) and type 1 (HIV-1) gag proteins are distinguishable by magnitude and breadth but not cellular phenotype |
| title | CD8+ T cell responses to human immunodeficiency viruses type 2 (HIV-2) and type 1 (HIV-1) gag proteins are distinguishable by magnitude and breadth but not cellular phenotype |
| title_full | CD8+ T cell responses to human immunodeficiency viruses type 2 (HIV-2) and type 1 (HIV-1) gag proteins are distinguishable by magnitude and breadth but not cellular phenotype |
| title_fullStr | CD8+ T cell responses to human immunodeficiency viruses type 2 (HIV-2) and type 1 (HIV-1) gag proteins are distinguishable by magnitude and breadth but not cellular phenotype |
| title_full_unstemmed | CD8+ T cell responses to human immunodeficiency viruses type 2 (HIV-2) and type 1 (HIV-1) gag proteins are distinguishable by magnitude and breadth but not cellular phenotype |
| title_short | CD8+ T cell responses to human immunodeficiency viruses type 2 (HIV-2) and type 1 (HIV-1) gag proteins are distinguishable by magnitude and breadth but not cellular phenotype |
| title_sort | cd8 t cell responses to human immunodeficiency viruses type 2 hiv 2 and type 1 hiv 1 gag proteins are distinguishable by magnitude and breadth but not cellular phenotype |
| work_keys_str_mv | AT gillespieg cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT pinheiros cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT sayeidaljameem cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT alabia cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT kayes cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT sargenjier cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT njaih cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT joofk cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT jayea cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT whittleh cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT rowlandjoness cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype AT dorrelll cd8tcellresponsestohumanimmunodeficiencyvirusestype2hiv2andtype1hiv1gagproteinsaredistinguishablebymagnitudeandbreadthbutnotcellularphenotype |