Isolation and preliminary characterization of gas1-1, a mutation causing partial suppression of the phenotype conferred by the gibberellin-insensitive (gai) mutation in Arabidopsis thaliana (L.) Heyhn.
The semi-dominant gai mutation of arabidopsis confers a dark-green dwarf phenotype resembling that of gibberellin (GA)-deficient mutants. In contrast to GA-deficient mutants, gai mutants do not respond to GA treatments and accumulate higher levels of bioactive GAs than are found in wild-type control...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1995
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| _version_ | 1797093981203464192 |
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| author | Carol, P Peng, J Harberd, N |
| author_facet | Carol, P Peng, J Harberd, N |
| author_sort | Carol, P |
| collection | OXFORD |
| description | The semi-dominant gai mutation of arabidopsis confers a dark-green dwarf phenotype resembling that of gibberellin (GA)-deficient mutants. In contrast to GA-deficient mutants, gai mutants do not respond to GA treatments and accumulate higher levels of bioactive GAs than are found in wild-type controls. The gai mutation thus alters the responses of plant cells to GA, indicating that the GAI (wild-type) gene product is involved in GA reception and/or signal transduction. Here we describe the isolation and preliminary characterization of a mutation, gas1-1, which is not linked to gai and which partially suppresses the effect of the gai mutation. Double mutant, gai gas1-1, homozygotes are less severely dwarfed and lighter green than gai GAS1 controls. However, comparisons of the effects of treatments with exogenous GA demonstrate that gas1-1 does not increase the GA responsiveness of the gai mutant. Thus the gas1-1 mutation appears to reduce the GA-dependency of plant growth, and identifies a gene (GAS1) whose product is a candidate GA signal-transduction component. |
| first_indexed | 2024-03-07T04:07:50Z |
| format | Journal article |
| id | oxford-uuid:c6d2a996-18b3-4bb6-b566-e4a9e6768fac |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:07:50Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | oxford-uuid:c6d2a996-18b3-4bb6-b566-e4a9e6768fac2022-03-27T06:40:35ZIsolation and preliminary characterization of gas1-1, a mutation causing partial suppression of the phenotype conferred by the gibberellin-insensitive (gai) mutation in Arabidopsis thaliana (L.) Heyhn.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c6d2a996-18b3-4bb6-b566-e4a9e6768facEnglishSymplectic Elements at Oxford1995Carol, PPeng, JHarberd, NThe semi-dominant gai mutation of arabidopsis confers a dark-green dwarf phenotype resembling that of gibberellin (GA)-deficient mutants. In contrast to GA-deficient mutants, gai mutants do not respond to GA treatments and accumulate higher levels of bioactive GAs than are found in wild-type controls. The gai mutation thus alters the responses of plant cells to GA, indicating that the GAI (wild-type) gene product is involved in GA reception and/or signal transduction. Here we describe the isolation and preliminary characterization of a mutation, gas1-1, which is not linked to gai and which partially suppresses the effect of the gai mutation. Double mutant, gai gas1-1, homozygotes are less severely dwarfed and lighter green than gai GAS1 controls. However, comparisons of the effects of treatments with exogenous GA demonstrate that gas1-1 does not increase the GA responsiveness of the gai mutant. Thus the gas1-1 mutation appears to reduce the GA-dependency of plant growth, and identifies a gene (GAS1) whose product is a candidate GA signal-transduction component. |
| spellingShingle | Carol, P Peng, J Harberd, N Isolation and preliminary characterization of gas1-1, a mutation causing partial suppression of the phenotype conferred by the gibberellin-insensitive (gai) mutation in Arabidopsis thaliana (L.) Heyhn. |
| title | Isolation and preliminary characterization of gas1-1, a mutation causing partial suppression of the phenotype conferred by the gibberellin-insensitive (gai) mutation in Arabidopsis thaliana (L.) Heyhn. |
| title_full | Isolation and preliminary characterization of gas1-1, a mutation causing partial suppression of the phenotype conferred by the gibberellin-insensitive (gai) mutation in Arabidopsis thaliana (L.) Heyhn. |
| title_fullStr | Isolation and preliminary characterization of gas1-1, a mutation causing partial suppression of the phenotype conferred by the gibberellin-insensitive (gai) mutation in Arabidopsis thaliana (L.) Heyhn. |
| title_full_unstemmed | Isolation and preliminary characterization of gas1-1, a mutation causing partial suppression of the phenotype conferred by the gibberellin-insensitive (gai) mutation in Arabidopsis thaliana (L.) Heyhn. |
| title_short | Isolation and preliminary characterization of gas1-1, a mutation causing partial suppression of the phenotype conferred by the gibberellin-insensitive (gai) mutation in Arabidopsis thaliana (L.) Heyhn. |
| title_sort | isolation and preliminary characterization of gas1 1 a mutation causing partial suppression of the phenotype conferred by the gibberellin insensitive gai mutation in arabidopsis thaliana l heyhn |
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