System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue
Improved systems and methods for the analysis of digital images are provided. More particularly, the present disclosure provides for improved systems and methods for the analysis of digital images of biological tissue samples. Exemplary embodiments provide for: i) segmenting, ii) grouping, and iii)...
| Main Authors: | , , , , , , , , , , |
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| 格式: | Patent |
| 出版: |
2015
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| _version_ | 1826295907934535680 |
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| author | Santamaria-Pang, A Rittscher, J Padfield, D Can, A Pang, Z Bello, M Ginty, F Sevinsky, C Li, Q Rothney, M Sarachan, B |
| author_facet | Santamaria-Pang, A Rittscher, J Padfield, D Can, A Pang, Z Bello, M Ginty, F Sevinsky, C Li, Q Rothney, M Sarachan, B |
| author_sort | Santamaria-Pang, A |
| collection | OXFORD |
| description | Improved systems and methods for the analysis of digital images are provided. More particularly, the present disclosure provides for improved systems and methods for the analysis of digital images of biological tissue samples. Exemplary embodiments provide for: i) segmenting, ii) grouping, and iii) quantifying molecular protein profiles of individual cells in terms of Sub cellular compartments (nuclei, membrane, and cytoplasm). The systems and methods of the present disclosure advantageously perform tissue segmentation at the Sub-cellular level to facilitate analyzing, grouping and quantifying protein expression profiles of tissue in tissue sections globally and/or locally. Performing local-global tissue analysis and protein quantification advantageously enables correlation of spatial and molecular configuration of cells with molecular information of different types of cancer. |
| first_indexed | 2024-03-07T04:08:11Z |
| format | Patent |
| id | oxford-uuid:c6ec4a93-ad53-4aee-b9ed-a45ea00b0e5d |
| institution | University of Oxford |
| last_indexed | 2024-03-07T04:08:11Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | oxford-uuid:c6ec4a93-ad53-4aee-b9ed-a45ea00b0e5d2022-03-27T06:41:22ZSystem and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissuePatenthttp://purl.org/coar/resource_type/c_1843uuid:c6ec4a93-ad53-4aee-b9ed-a45ea00b0e5dSymplectic Elements at Oxford2015Santamaria-Pang, ARittscher, JPadfield, DCan, APang, ZBello, MGinty, FSevinsky, CLi, QRothney, MSarachan, BImproved systems and methods for the analysis of digital images are provided. More particularly, the present disclosure provides for improved systems and methods for the analysis of digital images of biological tissue samples. Exemplary embodiments provide for: i) segmenting, ii) grouping, and iii) quantifying molecular protein profiles of individual cells in terms of Sub cellular compartments (nuclei, membrane, and cytoplasm). The systems and methods of the present disclosure advantageously perform tissue segmentation at the Sub-cellular level to facilitate analyzing, grouping and quantifying protein expression profiles of tissue in tissue sections globally and/or locally. Performing local-global tissue analysis and protein quantification advantageously enables correlation of spatial and molecular configuration of cells with molecular information of different types of cancer. |
| spellingShingle | Santamaria-Pang, A Rittscher, J Padfield, D Can, A Pang, Z Bello, M Ginty, F Sevinsky, C Li, Q Rothney, M Sarachan, B System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue |
| title | System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue |
| title_full | System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue |
| title_fullStr | System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue |
| title_full_unstemmed | System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue |
| title_short | System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue |
| title_sort | system and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue |
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