System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue

System and method for multiplexed biomarker quantitation using single cell segmentation on sequentially stained tissue

Improved systems and methods for the analysis of digital images are provided. More particularly, the present disclosure provides for improved systems and methods for the analysis of digital images of biological tissue samples. Exemplary embodiments provide for: i) segmenting, ii) grouping, and iii)...

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Bibliografiske detaljer
Main Authors:	Santamaria-Pang, A, Rittscher, J, Padfield, D, Can, A, Pang, Z, Bello, M, Ginty, F, Sevinsky, C, Li, Q, Rothney, M, Sarachan, B
Format:	Patent
Udgivet:	2015

Lignende værker

Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue.
af: Gerdes, M, et al.
Udgivet: (2013)

Cell segmentation and classification via unsupervised shape ranking
af: Santamaria-Pang, A, et al.
Udgivet: (2013)

Methods and algorithms for extracting high-content signatures from cells, tissues, and model organisms
af: Rittscher, J, et al.
Udgivet: (2011)

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af: Margolis, D, et al.
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af: Anup Sood, et al.
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af: Padfield, D, et al.
Udgivet: (2008)

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af: Padfield, D, et al.
Udgivet: (2011)

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af: Padfield, D, et al.
Udgivet: (2011)

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af: Maddalena M. Bolognesi, et al.
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af: Joshua Saylor, et al.
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af: Rittscher, A, et al.
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af: George Boguslawski, et al.
Udgivet: (2001-01-01)

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af: Zixia Zhou, et al.
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af: Miguel García-Hidalgo, et al.
Udgivet: (2024-01-01)

Mycobacterium tuberculosis PPD-induced immune biomarkers measurable in vitro following BCG vaccination of UK adolescents by multiplex bead array and intracellular cytokine staining
af: Smith, S, et al.
Udgivet: (2010)

Automated sequential chromogenic IHC double staining with two HRP substrates.
af: Kenneth Heesche Petersen, et al.
Udgivet: (2018-01-01)

Coupled minimum-cost flow cell tracking
af: Padfield, D, et al.
Udgivet: (2009)

Coupled minimum-cost flow cell tracking.
af: Padfield, D, et al.
Udgivet: (2009)

Methods for monitoring cellular motion and function
af: Padfield, D, et al.
Udgivet: (2008)

Rapid artefact removal and H&E-stained tissue segmentation
af: B. A. Schreiber, et al.
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af: Manish Kumar, et al.
Udgivet: (2016-10-01)

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af: Ahilya N. Sawh, et al.
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Sequential segmental analysis
af: Anderson Robert, et al.
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<it>Mycobacterium tuberculosis </it>PPD-induced immune biomarkers measurable <it>in vitro </it>following BCG vaccination of UK adolescents by multiplex bead array and intracellular cytokine staining
af: Worth Andrew, et al.
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af: Ann M. Lins, et al.
Udgivet: (1996-05-01)

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af: Tim Mandelkow, et al.
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af: Siqi Chen, et al.
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Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human
af: Oscar Maiques, et al.
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The Society for Immunotherapy of Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation
af: Ignacio I Wistuba, et al.
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Multiplexed Electrochemical Immunosensors for Clinical Biomarkers
af: Paloma Yáñez-Sedeño, et al.
Udgivet: (2017-04-01)

SHARD: an improved method for staining and visualizing multiplex immunofluorescence in optically cleared postmortem human brain tissue
af: Grace A. Rosen, et al.
Udgivet: (2024-10-01)

Sequential interactive image segmentation
af: Zheng Lin, et al.
Udgivet: (2023-07-01)

Effectiveness of dual sequential wavelength laser in the treatment of portwine stains – A retrospective study
af: Mohan Kumar Periyasamy, et al.
Udgivet: (2019-01-01)

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af: M. Guillaud, et al.
Udgivet: (1997-01-01)

Global probabilistic models for enhancing segmentation with convolutional networks
af: Fan, M, et al.
Udgivet: (2018)

Validation methods for cell cycle analysis algorithms in confocal fluorescence images
af: Padfield, D, et al.
Udgivet: (2006)

Spatio-temporal cell cycle phase analysis using level sets and fast marching methods.
af: Padfield, D, et al.
Udgivet: (2009)

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af: M.R. Arab, et al.
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af: M.R. Arab, et al.
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