Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion.
CD2 is a T lymphocyte glycoprotein that functions in adhesion of T lymphocytes and also as a putative receptor for activation signals. Functional data suggest that LFA-3, a widely distributed cell surface glycoprotein, may be the biological ligand of CD2. We have purified LFA-3 from human erythrocyt...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1987
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| _version_ | 1826295954288934912 |
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| author | Dustin, M Sanders, M Shaw, S Springer, T |
| author_facet | Dustin, M Sanders, M Shaw, S Springer, T |
| author_sort | Dustin, M |
| collection | OXFORD |
| description | CD2 is a T lymphocyte glycoprotein that functions in adhesion of T lymphocytes and also as a putative receptor for activation signals. Functional data suggest that LFA-3, a widely distributed cell surface glycoprotein, may be the biological ligand of CD2. We have purified LFA-3 from human erythrocytes and characterized the purified protein functionally. LFA-3 bound specifically to CD2+ cells, and this binding was inhibited by CD2 mAb. Conversely, purified LFA-3 inhibited binding of CD2 mAb to cells, and the concentration required for this effect suggests that LFA-3 half-saturated CD2 at 1-5 nM LFA-3. Purified LFA-3 inhibited rosetting of human and sheep erythrocytes with CD2+ T lymphoma cells and T lymphocytes, and mediated aggregation of a CD2+ T lymphoma cell line. Purified LFA-3 reconstituted into planar membranes mediated efficient CD2-dependent adhesion of T lymphoblasts. These data demonstrate that LFA-3 is a ligand for CD2 and that LFA-3 can mediate T lymphocyte adhesion. |
| first_indexed | 2024-03-07T04:08:54Z |
| format | Journal article |
| id | oxford-uuid:c7288d7d-1cb4-47bb-84c5-b6e67bda9175 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:08:54Z |
| publishDate | 1987 |
| record_format | dspace |
| spelling | oxford-uuid:c7288d7d-1cb4-47bb-84c5-b6e67bda91752022-03-27T06:43:02ZPurified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c7288d7d-1cb4-47bb-84c5-b6e67bda9175EnglishSymplectic Elements at Oxford1987Dustin, MSanders, MShaw, SSpringer, TCD2 is a T lymphocyte glycoprotein that functions in adhesion of T lymphocytes and also as a putative receptor for activation signals. Functional data suggest that LFA-3, a widely distributed cell surface glycoprotein, may be the biological ligand of CD2. We have purified LFA-3 from human erythrocytes and characterized the purified protein functionally. LFA-3 bound specifically to CD2+ cells, and this binding was inhibited by CD2 mAb. Conversely, purified LFA-3 inhibited binding of CD2 mAb to cells, and the concentration required for this effect suggests that LFA-3 half-saturated CD2 at 1-5 nM LFA-3. Purified LFA-3 inhibited rosetting of human and sheep erythrocytes with CD2+ T lymphoma cells and T lymphocytes, and mediated aggregation of a CD2+ T lymphoma cell line. Purified LFA-3 reconstituted into planar membranes mediated efficient CD2-dependent adhesion of T lymphoblasts. These data demonstrate that LFA-3 is a ligand for CD2 and that LFA-3 can mediate T lymphocyte adhesion. |
| spellingShingle | Dustin, M Sanders, M Shaw, S Springer, T Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion. |
| title | Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion. |
| title_full | Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion. |
| title_fullStr | Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion. |
| title_full_unstemmed | Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion. |
| title_short | Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion. |
| title_sort | purified lymphocyte function associated antigen 3 binds to cd2 and mediates t lymphocyte adhesion |
| work_keys_str_mv | AT dustinm purifiedlymphocytefunctionassociatedantigen3bindstocd2andmediatestlymphocyteadhesion AT sandersm purifiedlymphocytefunctionassociatedantigen3bindstocd2andmediatestlymphocyteadhesion AT shaws purifiedlymphocytefunctionassociatedantigen3bindstocd2andmediatestlymphocyteadhesion AT springert purifiedlymphocytefunctionassociatedantigen3bindstocd2andmediatestlymphocyteadhesion |