Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus.
Inter- and intra-observer variation was examined among six microscopists who read 50 scrub typhus (ST) and murine typhus (MT) indirect immunofluorescence assay (IFA) immunoglobulin M (IgM) slides. Inter-observer agreement was moderate (κ = 0.45) for MT and fair (κ = 0.32) for ST, and was significant...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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| _version_ | 1797094120376762368 |
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| author | Phetsouvanh, R Thojaikong, T Phoumin, P Sibounheuang, B Phommasone, K Chansamouth, V Lee, S Newton, P Blacksell, S |
| author_facet | Phetsouvanh, R Thojaikong, T Phoumin, P Sibounheuang, B Phommasone, K Chansamouth, V Lee, S Newton, P Blacksell, S |
| author_sort | Phetsouvanh, R |
| collection | OXFORD |
| description | Inter- and intra-observer variation was examined among six microscopists who read 50 scrub typhus (ST) and murine typhus (MT) indirect immunofluorescence assay (IFA) immunoglobulin M (IgM) slides. Inter-observer agreement was moderate (κ = 0.45) for MT and fair (κ = 0.32) for ST, and was significantly correlated with experience (P = 0.03 and P = 0.004, respectively); κ-scores for intra-observer agreement between morning and afternoon readings (range = 0.35-0.86) were not correlated between years of experience for ST and MT IFAs (Spearman's ρ = 0.31, P = 0.54 and P = 0.14, respectively; P = 0.78). Storage at 4°C for 2 days showed a change from positive to negative in 20-32% of slides. Although the titers did not dramatically change after 14 days of storage, the final interpretation (positive to negative) did change in 36-50% of samples, and it, therefore, recommended that slides should be read as soon as possible after processing. |
| first_indexed | 2024-03-07T04:09:44Z |
| format | Journal article |
| id | oxford-uuid:c76cc0e0-ce4b-416a-8fcc-0219fa7a57ab |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:09:44Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:c76cc0e0-ce4b-416a-8fcc-0219fa7a57ab2022-03-27T06:44:55ZInter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c76cc0e0-ce4b-416a-8fcc-0219fa7a57abEnglishSymplectic Elements at Oxford2013Phetsouvanh, RThojaikong, TPhoumin, PSibounheuang, BPhommasone, KChansamouth, VLee, SNewton, PBlacksell, SInter- and intra-observer variation was examined among six microscopists who read 50 scrub typhus (ST) and murine typhus (MT) indirect immunofluorescence assay (IFA) immunoglobulin M (IgM) slides. Inter-observer agreement was moderate (κ = 0.45) for MT and fair (κ = 0.32) for ST, and was significantly correlated with experience (P = 0.03 and P = 0.004, respectively); κ-scores for intra-observer agreement between morning and afternoon readings (range = 0.35-0.86) were not correlated between years of experience for ST and MT IFAs (Spearman's ρ = 0.31, P = 0.54 and P = 0.14, respectively; P = 0.78). Storage at 4°C for 2 days showed a change from positive to negative in 20-32% of slides. Although the titers did not dramatically change after 14 days of storage, the final interpretation (positive to negative) did change in 36-50% of samples, and it, therefore, recommended that slides should be read as soon as possible after processing. |
| spellingShingle | Phetsouvanh, R Thojaikong, T Phoumin, P Sibounheuang, B Phommasone, K Chansamouth, V Lee, S Newton, P Blacksell, S Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus. |
| title | Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus. |
| title_full | Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus. |
| title_fullStr | Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus. |
| title_full_unstemmed | Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus. |
| title_short | Inter- and intra-operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus. |
| title_sort | inter and intra operator variability in the reading of indirect immunofluorescence assays for the serological diagnosis of scrub typhus and murine typhus |
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