Survival of Burkholderia pseudomallei in distilled water for 16 years
Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10 cells/ml and 1.4×10...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Published: |
2011
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| Summary: | Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10 cells/ml and 1.4×10 cells/ml, respectively. A colony count on agar of 1.0×10 cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement. © 2011 Royal Society of Tropical Medicine and Hygiene. |
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