Survival of Burkholderia pseudomallei in distilled water for 16 years
Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10 cells/ml and 1.4×10...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Published: |
2011
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| _version_ | 1797094335350571008 |
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| author | Pumpuang, A Chantratita, N Wikraiphat, C Saiprom, N Peacock, S Day, N Wuthiekanun, V |
| author_facet | Pumpuang, A Chantratita, N Wikraiphat, C Saiprom, N Peacock, S Day, N Wuthiekanun, V |
| author_sort | Pumpuang, A |
| collection | OXFORD |
| description | Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10 cells/ml and 1.4×10 cells/ml, respectively. A colony count on agar of 1.0×10 cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement. © 2011 Royal Society of Tropical Medicine and Hygiene. |
| first_indexed | 2024-03-07T04:12:42Z |
| format | Journal article |
| id | oxford-uuid:c8609f1a-21b1-4b28-968a-9bbdc1bee45c |
| institution | University of Oxford |
| last_indexed | 2024-03-07T04:12:42Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:c8609f1a-21b1-4b28-968a-9bbdc1bee45c2022-03-27T06:51:43ZSurvival of Burkholderia pseudomallei in distilled water for 16 yearsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c8609f1a-21b1-4b28-968a-9bbdc1bee45cSymplectic Elements at Oxford2011Pumpuang, AChantratita, NWikraiphat, CSaiprom, NPeacock, SDay, NWuthiekanun, VBurkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10 cells/ml and 1.4×10 cells/ml, respectively. A colony count on agar of 1.0×10 cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement. © 2011 Royal Society of Tropical Medicine and Hygiene. |
| spellingShingle | Pumpuang, A Chantratita, N Wikraiphat, C Saiprom, N Peacock, S Day, N Wuthiekanun, V Survival of Burkholderia pseudomallei in distilled water for 16 years |
| title | Survival of Burkholderia pseudomallei in distilled water for 16 years |
| title_full | Survival of Burkholderia pseudomallei in distilled water for 16 years |
| title_fullStr | Survival of Burkholderia pseudomallei in distilled water for 16 years |
| title_full_unstemmed | Survival of Burkholderia pseudomallei in distilled water for 16 years |
| title_short | Survival of Burkholderia pseudomallei in distilled water for 16 years |
| title_sort | survival of burkholderia pseudomallei in distilled water for 16 years |
| work_keys_str_mv | AT pumpuanga survivalofburkholderiapseudomalleiindistilledwaterfor16years AT chantratitan survivalofburkholderiapseudomalleiindistilledwaterfor16years AT wikraiphatc survivalofburkholderiapseudomalleiindistilledwaterfor16years AT saipromn survivalofburkholderiapseudomalleiindistilledwaterfor16years AT peacocks survivalofburkholderiapseudomalleiindistilledwaterfor16years AT dayn survivalofburkholderiapseudomalleiindistilledwaterfor16years AT wuthiekanunv survivalofburkholderiapseudomalleiindistilledwaterfor16years |