Mining the active proteome in plant science and biotechnology.
Protein activity is essential functional information, yet difficult to predict from transcript or protein data. Activity-based protein profiling (ABPP) displays active proteins in proteomes using small molecule probes that irreversibly label proteins in their active state. Here, we review proof-of-c...
| Main Authors: | , |
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| Format: | Journal article |
| Jezik: | English |
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2010
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| _version_ | 1826296206931787776 |
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| author | Kołodziejek, I van der Hoorn, R |
| author_facet | Kołodziejek, I van der Hoorn, R |
| author_sort | Kołodziejek, I |
| collection | OXFORD |
| description | Protein activity is essential functional information, yet difficult to predict from transcript or protein data. Activity-based protein profiling (ABPP) displays active proteins in proteomes using small molecule probes that irreversibly label proteins in their active state. Here, we review proof-of-concept ABPP studies in plant science. These studies displayed activities of dozens of plant cysteine proteases, lipases, methylesterases and the proteasome. ABPP in plants revealed differential protein activities in development and immunity and uncovered striking selectivity of pathogen-derived inhibitors and unexpected targets of commercial inhibitors. The unique, high-content information of ABPP and the robustness and simplicity of the assays will make ABPP a powerful tool in future plant science and biotechnology. |
| first_indexed | 2024-03-07T04:12:46Z |
| format | Journal article |
| id | oxford-uuid:c8659ac1-56f2-43f5-a0d6-32cc60e91996 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:12:46Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:c8659ac1-56f2-43f5-a0d6-32cc60e919962022-03-27T06:51:47ZMining the active proteome in plant science and biotechnology.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c8659ac1-56f2-43f5-a0d6-32cc60e91996EnglishSymplectic Elements at Oxford2010Kołodziejek, Ivan der Hoorn, RProtein activity is essential functional information, yet difficult to predict from transcript or protein data. Activity-based protein profiling (ABPP) displays active proteins in proteomes using small molecule probes that irreversibly label proteins in their active state. Here, we review proof-of-concept ABPP studies in plant science. These studies displayed activities of dozens of plant cysteine proteases, lipases, methylesterases and the proteasome. ABPP in plants revealed differential protein activities in development and immunity and uncovered striking selectivity of pathogen-derived inhibitors and unexpected targets of commercial inhibitors. The unique, high-content information of ABPP and the robustness and simplicity of the assays will make ABPP a powerful tool in future plant science and biotechnology. |
| spellingShingle | Kołodziejek, I van der Hoorn, R Mining the active proteome in plant science and biotechnology. |
| title | Mining the active proteome in plant science and biotechnology. |
| title_full | Mining the active proteome in plant science and biotechnology. |
| title_fullStr | Mining the active proteome in plant science and biotechnology. |
| title_full_unstemmed | Mining the active proteome in plant science and biotechnology. |
| title_short | Mining the active proteome in plant science and biotechnology. |
| title_sort | mining the active proteome in plant science and biotechnology |
| work_keys_str_mv | AT kołodziejeki miningtheactiveproteomeinplantscienceandbiotechnology AT vanderhoornr miningtheactiveproteomeinplantscienceandbiotechnology |