Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro.

The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-b...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser: Siritantikorn, S, Jintaworn, S, Noisakran, S, Suputtamongkol, Y, Paris, D, Blacksell, S
Format: Journal article
Sprache:English
Veröffentlicht: 2012
Beschreibung
Zusammenfassung:The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.