Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro.

The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-b...

Ամբողջական նկարագրություն

Մատենագիտական մանրամասներ
Հիմնական հեղինակներ: Siritantikorn, S, Jintaworn, S, Noisakran, S, Suputtamongkol, Y, Paris, D, Blacksell, S
Ձևաչափ: Journal article
Լեզու:English
Հրապարակվել է: 2012
Նկարագրություն
Ամփոփում:The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.