Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics.
© 2015 The Authors. Centrosomes are major microtubule organising centres comprising a pair of centrioles surrounded by pericentriolar material (PCM). The PCM expands dramatically as cells enter mitosis, and we previously showed that two key PCM components, Centrosomin (Cnn) and Spd-2, cooperate to f...
| Asıl Yazarlar: | , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
2015
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| _version_ | 1826296372686487552 |
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| author | Conduit, P Raff, J |
| author_facet | Conduit, P Raff, J |
| author_sort | Conduit, P |
| collection | OXFORD |
| description | © 2015 The Authors. Centrosomes are major microtubule organising centres comprising a pair of centrioles surrounded by pericentriolar material (PCM). The PCM expands dramatically as cells enter mitosis, and we previously showed that two key PCM components, Centrosomin (Cnn) and Spd-2, cooperate to form a scaffold structure around the centrioles that recruits the mitotic PCM in Drosophila; the SPD-5 and SPD-2 proteins appear to play a similar function in C. Elegans [1-3]. In fly syncytial embryos, Cnn and Spd-2 are initially recruited into a central region of the PCM and then flux outwards [4-6]. This centrosomal flux is potentially important, but it has so far not been reported in any other cell type. Here we examine the dynamic behaviour of Cnn and Spd-2 in Drosophila larval brain cells. Spd-2 fluxes outwards from the centrioles in both brains and embryos in a microtubule-independent manner. In contrast, although Cnn is initially incorporated into the region of the PCM occupied by Spd-2 in both brains and embryos, Cnn fluxes outwards along microtubules in embryos, but not in brain cells, where it remains concentrated around the centrosomal Spd-2. Thus, the microtubule-independent centrosomal-flux of Spd-2 occurs in multiple fly cell types, while the microtubule-dependent outward flux of Cnn appears to be restricted to the syncytial embryo. |
| first_indexed | 2024-03-07T04:15:19Z |
| format | Journal article |
| id | oxford-uuid:c937a2db-5d07-4fa3-b314-b5e098af0155 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:15:19Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | oxford-uuid:c937a2db-5d07-4fa3-b314-b5e098af01552022-03-27T06:57:33ZDifferent Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:c937a2db-5d07-4fa3-b314-b5e098af0155EnglishSymplectic Elements at Oxford2015Conduit, PRaff, J© 2015 The Authors. Centrosomes are major microtubule organising centres comprising a pair of centrioles surrounded by pericentriolar material (PCM). The PCM expands dramatically as cells enter mitosis, and we previously showed that two key PCM components, Centrosomin (Cnn) and Spd-2, cooperate to form a scaffold structure around the centrioles that recruits the mitotic PCM in Drosophila; the SPD-5 and SPD-2 proteins appear to play a similar function in C. Elegans [1-3]. In fly syncytial embryos, Cnn and Spd-2 are initially recruited into a central region of the PCM and then flux outwards [4-6]. This centrosomal flux is potentially important, but it has so far not been reported in any other cell type. Here we examine the dynamic behaviour of Cnn and Spd-2 in Drosophila larval brain cells. Spd-2 fluxes outwards from the centrioles in both brains and embryos in a microtubule-independent manner. In contrast, although Cnn is initially incorporated into the region of the PCM occupied by Spd-2 in both brains and embryos, Cnn fluxes outwards along microtubules in embryos, but not in brain cells, where it remains concentrated around the centrosomal Spd-2. Thus, the microtubule-independent centrosomal-flux of Spd-2 occurs in multiple fly cell types, while the microtubule-dependent outward flux of Cnn appears to be restricted to the syncytial embryo. |
| spellingShingle | Conduit, P Raff, J Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics. |
| title | Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics. |
| title_full | Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics. |
| title_fullStr | Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics. |
| title_full_unstemmed | Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics. |
| title_short | Different Drosophila cell types exhibit differences in mitotic centrosome assembly dynamics. |
| title_sort | different drosophila cell types exhibit differences in mitotic centrosome assembly dynamics |
| work_keys_str_mv | AT conduitp differentdrosophilacelltypesexhibitdifferencesinmitoticcentrosomeassemblydynamics AT raffj differentdrosophilacelltypesexhibitdifferencesinmitoticcentrosomeassemblydynamics |